Proteomic Profiling of HDL in Newly Diagnosed Breast Cancer Based on Tumor Molecular Classification and Clinical Stage of Disease

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de Fatima Mello Santana, Monique ; Sawada, Maria Isabela Bloise Alves Caldas ; Souza Junior, Douglas Ricardo ; Giacaglia, Marcia Benacchio ; Reis, Mozania ; Xavier, Jacira ; Correa-Giannella, Maria Lucia ; Soriano, Francisco Garcia ; Gebrim, Luiz Henrique ; Ronsein, Graziella Eliza ; Passarelli, Marisa

• Tipo de documento: Artigo Científico
• Fonte: CELLS; v. 13, n. 16, p. 19-pg., 2024-08-01.

Resumo

The association between high-density lipoprotein (HDL) cholesterol and breast cancer (BC) remains controversial due to the high complexity of the HDL particle and its functionality. The HDL proteome was determined in newly diagnosed BC classified according to the molecular type [luminal A or B (LA or LB), HER2, and triple-negative (TN)] and clinical stage of the disease. Women (n = 141) aged between 18 and 80 years with BC, treatment-na & iuml;ve, and healthy women [n = 103; control group (CT)], matched by age and body mass index, were included. Data-independent acquisition (DIA) proteomics was performed in isolated HDL (D = 1.063-1.21 g/mL). Results: Paraoxonase1, carnosine dipeptidase1, immunoglobulin mMu heavy chain constant region (IGHM), apoA-4, and transthyretin were reduced, and serum amyloid A2 and tetranectin were higher in BC compared to CT. In TNBC, apoA-1, apoA-2, apoC-2, and apoC-4 were reduced compared to LA, LB, and HER2, and apoA-4 compared to LA and HER2. ComplementC3, lambda immunoglobulin2/3, serpin3, IGHM, complement9, alpha2 lysine rich-glycoprotein1, and complement4B were higher in TNBC in comparison to all other types; complement factor B and vitamin D-binding protein were in contrast to LA and HER2, and plasminogen compared to LA and LB. In grouped stages III + IV, tetranectin and alpha2-macroglobulin were reduced, and haptoglobin-related protein; lecithin cholesterol acyltransferase, serum amyloid A1, and IGHM were increased compared to stages I + II. Conclusions: A differential proteomic profile of HDL in BC based on tumor molecular classification and the clinical stage of the disease may contribute to a better understanding of the association of HDL with BC pathophysiology, treatment, and outcomes. (AU)

• Processo FAPESP: 19/18431-4 - Lipoproteínas de densidade alta, produtos de glicação avançada e óxidos de colesterol no Câncer de Mama: associação com a classificação molecular do tumor e o prognóstico da doença
• Beneficiário: Marisa Passarelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/25702-4 - Compreendendo os efeitos da lipoproteína de alta densidade em macrófagos humanos
• Beneficiário: Douglas Ricardo de Souza Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 21/02401-9 - Atividade anti-inflamatória e transporte de microRNA pelas lipoproteínas de densidade alta (HDL) no Câncer de Mama: associação com o prognóstico da doença predito pela classificação molecular do tumor
• Beneficiário: Monique de Fatima Mello Santana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/15603-0 - Desvendando mecanismos envolvidos no controle glicêmico e nas complicações crônicas do Diabetes mellitus: contribuições à saúde humana
• Beneficiário: Ubiratan Fabres Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 23/00995-4 - Desenvolvimento de métricas de proteômica quantitativa capazes de capturar a complexidade funcional e composicional da lipoproteína de alta densidade
• Beneficiário: Graziella Eliza Ronsein
• Modalidade de apoio: Auxílio à Pesquisa - Regular