Paternal exercise induces antioxidant defenses by α-Klotho/Keap1 pathways in the skeletal muscle of offspring exposed to a high fat-diet without changing telomere length

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Neto, Ivo Vieira de Sousa ; Pinto, Ana Paula ; Andrade, Rosangela Vieira de ; de Souza, Fabiane Hiratsuka Veiga ; de Souza, Paulo Eduardo Narcizo ; Assis, Victoria ; Tibana, Ramires Alsamir ; Neves, Rodrigo Vanerson Passos ; Rosa, Thiago Santos ; Prestes, Jonato ; da Silva, Adelino Sanchez Ramos ; Marqueti, Rita de Cassia

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 134, p. 15-pg., 2024-09-23.

Resumo

Although previous studies demonstrated that the ancestral lifestyle can enhance the metabolic health of offspring exposed to an obesogenic diet, the specific connections between these positive effects in redox state and telomere length are unknown. We investigated the impact of paternal resistance training (RT) on stress-responsive signaling and the pathways involved in telomere homeostasis in skeletal muscle. This investigation encompassed both the fathers and first-generation litter exposed to a long-term standard diet (24 weeks) and high fat diet (HFD). Wistar rats were randomized into sedentary or trained fathers (8 weeks of resistance training). The offspring were obtained by mating with sedentary females. Upon weaning, male offspring were divided into four groups: offspring of sedentary or trained fathers exposed to either a control diet or HFD. The gastrocnemius was prepared for reverse transcription-quantitative polymerase chain reaction, immunoblotting, ELISA, and electron paramagnetic resonance spectroscopy. RT upregulated shelterin mRNA levels and antioxidant protein, preserving muscle telomere in fathers. Conversely, HFD induced a disturbance in the redox balance, which may have contributed to the offspring telomere shortening from sedentary fathers. Preconceptional paternal RT downregulates Kelch-like ECH-associated protein 1 ( Keap1) mRNA levels in the skeletal muscle of progeny exposed to HFD, driving an increase in Glutathione reductase mRNA levels, Sod1 and Catalase protein levels to mitigate ROS production. Also, paternal exercise upregulates alpha-Klotho protein levels, mediating antioxidative responses without altering shelterin mRNA levels and telomere length. We provide the first in-depth analysis that the offspring's redox state seems to be directly associated with the beneficial effects of paternal exercise. (c) 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies. (AU)

• Processo FAPESP: 21/06291-3 - EMU concedido no processo 2019/11820-5: ChemiDoc Imaging System e acessórios
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• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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