| Texto completo | |
| Autor(es): |
Navarro, Claudia D. C.
;
Francisco, Annelise
;
Costa, Ericka F. D.
;
Costa, Ana P. Dalla
;
Sartori, Marina R.
;
Bizerra, Paulo F. V.
;
Salgado, Andreia R.
;
Figueira, Tiago R.
;
Vercesi, Anibal E.
;
Castilho, Roger F.
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Experimental Gerontology; v. 193, p. 12-pg., 2024-05-25. |
| Resumo | |
Overall health relies on features of skeletal muscle that generally decline with age, partly due to mechanisms associated with mitochondrial redox imbalance and bioenergetic dysfunction. Previously, aged mice genetically devoid of the mitochondrial NAD(P) + transhydrogenase (NNT, encoded by the nicotinamide nucleotide transhydrogenase gene), an enzyme involved in mitochondrial NADPH supply, were shown to exhibit deficits in locomotor behavior. Here, by using young, middle-aged, and older NNT-deficient ( Nnt - / - ) mice and age -matched controls ( Nnt + / + ), we aimed to investigate how muscle bioenergetic function and motor performance are affected by NNT expression and aging. Mice were subjected to the wire -hang test to assess locomotor performance, while mitochondrial bioenergetics was evaluated in fiber bundles from the soleus, vastus lateralis and plantaris muscles. An age -related decrease in the average wire -hang score was observed in middle-aged and older Nnt - / - mice compared to age -matched controls. Although respiratory rates in the soleus, vastus lateralis and plantaris muscles did not significantly differ between the genotypes in young mice, the rates of oxygen consumption did decrease in the soleus and vastus lateralis muscles of middle-aged and older Nnt - / - mice. Notably, the soleus, which exhibited the highest NNT expression level, was the muscle most affected by aging, and NNT loss. Additionally, histology of the soleus fibers revealed increased numbers of centralized nuclei in older Nnt - / - mice, indicating abnormal morphology. In summary, our findings suggest that NNT expression deficiency causes locomotor impairments and muscle dysfunction during aging in mice. (AU) | |
| Processo FAPESP: | 19/20855-7 - Papel da NAD(P)-Transidrogenase mitocondrial no desenvolvimento da Doença Hepática Gordurosa e no envelhecimento |
| Beneficiário: | Claudia Daniele Carvalho Navarro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 23/00229-0 - Papéis fisiopatológicos da NAD(P)+ transidrogenase mitocondrial |
| Beneficiário: | Roger Frigério Castilho |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 20/05202-4 - Papel da NAD(P)+ transidrogenase mitocondrial na neurotransmissão monoaminérgica e neurodegeneração em camundongos |
| Beneficiário: | Annelise Francisco |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/17728-8 - Função e disfunção mitocondrial: implicações para o envelhecimento e doenças associadas |
| Beneficiário: | Aníbal Eugênio Vercesi |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 23/06469-2 - Papel da NAD(P)+ transidrogenase (NNT) na inflamação hipotalâmica e biogênese do tecido adiposo marrom em camundongos |
| Beneficiário: | Ericka Francislaine Dias Costa |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |