Long-Term Exogenous Melatonin Treatment Modulates Overall Feed Efficiency and Protects Ovarian Tissue Against Injuries Caused by Ethanol-Induced Oxidative Stress in Adult UChB Rats

Autor(es):Mostrar menos -

Chuffa, Luiz Gustavo A. ^[1] ; Amorim, Joao P. A. ^[1] ; Teixeira, Giovana R. ^[2] ; Mendes, Leonardo O. ^[1] ; Fioruci, Beatriz A. ^[1] ; Pinheiro, Patricia F. F. ^[2] ; Seiva, Fabio R. F. ^[3] ; Novelli, Ethel L. B. ^[3] ; de Mello Junior, Wilson ^[2] ; Martinez, Marcelo ^[4] ; Almeida-Francia, Camila C. D. ^[2] ; Martinez, Francisco E. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Struct & Cell Biol Program, Inst Biol, UNICAMP, Campinas, SP - Brazil

^[2] Univ Estadual Paulista, Dept Anat, Inst Biosci, UNESP, BR-18618000 Botucatu, SP - Brazil

^[3] Univ Estadual Paulista, Dept Chem & Biochem, Inst Biosci, UNESP, BR-18618000 Botucatu, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Morphol & Pathol, BR-13560 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH; v. 35, n. 8, p. 1498-1508, AUG 2011.
• Citações Web of Science: 20

Resumo

Background: Chronic ethanol intake leads to reproductive damage including reactive oxygen species formation, which accelerates the oxidative process. Melatonin is known to regulate the reproductive cycle, food/liquid intake, and it may also act as a potent antioxidant indoleamine. The aim of this study was to verify the effects of alcoholism and melatonin treatment on overall feed efficiency and to analyze its protective role against the oxidative stress in the ovarian tissue of UChB rats (submitted to 10% {[}v/v] voluntary ethanol consumption). Methods: Forty adult female rats (n = 10/group) were finally selected for this study: UChB Co: drinking water only; and UChB EtOH: drinking ethanol at 2 to 6 ml/100 g/d + water, both receiving 0.9% NaCl + 95% ethanol 0.04 ml as vehicle. Concomitantly, UChB Co + M and UChB EtOH + M groups were infused with vehicle + melatonin (100 mu g/100 g body weight/d) intraperitoneally over 60 days. All animals were euthanized by decapitation during the morning estrus (4 AM). Results: Body weight gain was reduced with ethanol plus melatonin after 40 days of treatment. In both melatonin-treated groups, it was observed a reduction in food-derived calories and liquid intake toward the end of treatment. The amount of consumed ethanol dropped during the treatment. Estrous cycle was longer in rats that received both ethanol and melatonin, with prolonged diestrus. Following to oxidative status, lipid hydroperoxide levels were higher in the ovaries of ethanol-preferring rats and decreased after melatonin treatment. Additionally, antioxidant activities of superoxide dismutase, glutathione peroxidase activity, and glutathione reductase activity were increased in melatonin-treated groups. Conclusions: We suggest that melatonin is able to affect feed efficiency and, conversely, it protects the ovaries against the oxidative stress arising from ethanol consumption. (AU)

• Processo FAPESP: 08/56229-8 - Caracterização do comportamento materno e suas implicações no desenvolvimento físico, sexual inicial, reprodutivo e hormonal da prole feminina de ratas UChA e UChB (consumidoras voluntárias de etanol a 10%)
• Beneficiário: Francisco Eduardo Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/59967-7 - Ação da melatonina sobre os receptores estrogênicos ER alfa e ER beta e progestagênicos PRA e PRB nos órgãos genitais femininos internos e o estresse oxidativo nos ovários de ratas adultas UChB (consumidoras voluntárias de etanol a 10%)
• Beneficiário: Luiz Gustavo de Almeida Chuffa
• Modalidade de apoio: Bolsas no Brasil - Doutorado