| Texto completo | |
| Autor(es): |
Andrade, Livia Mayra
;
Guilherme, Ederson Paulo Xavier
;
Hijo, Ariel Antonio Campos Toledo
;
Bruziquesi, Carlos Giovani Oliveira
;
Brandi, Igor Viana
;
Lima, William James Nogueira
;
de Carvalho, Gleidson Giordano Pinto
;
Mesquita, Bruna Mara Aparecida de Carvalho
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES; v. 100, p. 9-pg., 2025-01-02. |
| Resumo | |
Whey proteins are important source of bioactive peptides, which can present antioxidant, anti-hypertensive, and antimicrobial activities among other effects. Indeed, whey proteins have valuable nutritional importance, including potential immunological and antiviral related effects. However, to design protein formulations for a target application its extraction and purification is essential. In this context, the ionic liquid (IL) technology combined with the magnetic nanotechnology may constitute an interesting sustainable alternative. In this work, a magnetic IL-based adsorbent (MNPs-IL-Ni2+) was produced and its ability for capturing whey proteins was investigated. The impact of pH, temperature, and NaCl concentration on protein adsorption was systematically evaluated using bovine serum albumin (BSA) as a model protein and the magnetic IL-based adsorbent (MNPs-ILNi2+) through a design of experiments (DoE) approach. Then, whey proteins were recovered from milk whey under fixed process conditions. A second order model was adjusted with coefficient of determination of 94.51 %. Increasing of temperature and NaCl concentration affected positively protein adsorption. Otherwise, pH values lower than 5 were more favorable to the protein adsorption. Interestingly, alpha-lactalbumin (alpha-LA), betalactoglobulin ((3-LG), immunoglobulin (IgG), BSA and lactoferrin (LF) were recovered from milk whey by using IL-based adsorbent, which was evidenced by SDS-PAGE analysis. Therefore, the IL-based adsorbent is a promising tool for whey protein extraction and has high potential application in the agri-food industry. (AU) | |
| Processo FAPESP: | 19/18608-1 - Desenvolvimento e aplicação de líquidos iônicos derivados de compostos bioativos e de origem natural como aditivos funcionais e nutracêuticos para a indústria de alimentos |
| Beneficiário: | Ariel Antonio Campos Toledo Hijo |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 22/04614-2 - Simulação da digestão de sistemas compostos por líquidos iônicos para o desenvolvimento de aditivos alimentares funcionais |
| Beneficiário: | Ariel Antonio Campos Toledo Hijo |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |