| Texto completo | |
| Autor(es): |
Nobrega, Guilherme M.
;
Mccoll, Eliza R.
;
Antolini-Tavares, Arthur
;
Souza, Renato T.
;
Cecatti, Jose Guilherme
;
Costa, Maria Laura
;
Mysorekar, Indira U.
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | American Journal of Reproductive Immunology; v. 93, n. 1, p. 7-pg., 2025-01-01. |
| Resumo | |
ProblemCOVID-19 during pregnancy is linked to increased maternal morbidity and a higher incidence of preterm births (PTBs), yet the underlying mechanisms remain unclear. Cellular senescence, characterized by the irreversible cessation of cell division, is a critical process in placental function, and its dysregulation has been implicated in pregnancy complications like PTB. Senescence can be induced by various stressors, including oxidative stress, DNA damage, and viral infections.Method of StudyIn this study, we determined whether COVID-19 had an impact on placental senescence. We examined placentas from women infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) (n = 10 term, 4 preterm) compared to uninfected controls (n = 10 term, 3 preterm). The placentas were analyzed for SARS-CoV-2 infection (spike and nucleocapsid viral proteins), markers of DNA damage (gamma H2AX) and oxidative stress (ROS), and senescence (telomere length, cell cycle regulators, and senescence-associated secretory phenotype [SASP]).ResultsAlthough no overall differences in cellular senescence markers were observed between the COVID-19 positive and negative groups, we found increased secreted SASP markers. Confocal microscopy of placentas from COVID-19 positive cases revealed localized areas of oxidative stress and DNA damage colocalized with SARS-CoV-2 spike protein.ConclusionsThese findings indicate that SARS-CoV-2 infection induces localized focal placental damage, warranting further investigation into its impact on maternal and perinatal outcomes. (AU) | |
| Processo FAPESP: | 21/09937-1 - COVID-19 e pré-eclâmpsia: causa ou consequência? |
| Beneficiário: | Maria Laura Costa do Nascimento |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |