| Texto completo | |
| Autor(es): |
Justino, Isabela Araujo
;
Furlan, Joao Pedro Rueda
;
Ferreira, Iasmin Rosane Silva
;
Marincek, Andreia
;
Aldana-Mejia, Jennyfer A.
;
Tucci, Luiz Fernando Fortunato
;
Bastos, Jairo Kenupp
;
Stehling, Eliana Guedes
;
Marzocchi-Machado, Cleni M.
;
Marcato, Priscyla Daniely
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PROCESSES; v. 12, n. 12, p. 26-pg., 2024-12-01. |
| Resumo | |
This study evaluates the antimicrobial, antioxidant, and anticancer effects of Brazilian red propolis extract (BRPE) and its nanoencapsulated form (NCBRPE) to address bacteria and conditions associated with the ovarian cancer microenvironment. The NCBRPE showed an average size of 178.3 +/- 3.3 nm, a polydispersity index (PdI) of 0.06, and an encapsulation efficiency exceeding 97% for the main bioactive compounds of propolis. Antimicrobial assays revealed that BRPE exhibited minimum inhibitory concentrations (MICs) ranging from 4 to 256 mg/L against seven bacterial strains, while NCBRPE demonstrated sustained efficacy, with a biofilm inhibitory concentration (BIC) of 128 mg/L against Burkholderia cepacia. In clonogenic assays, NCBRPE reduced long-term cancer cell proliferation, achieving a 10-fold decrease in colony formation compared to untreated controls, significantly outperforming BRPE. Flow cytometry indicated that NCBRPE induced apoptosis in 29% +/- 0.4 of ovarian cancer cells (OVCAR-3). Additionally, the DPPH assay confirmed the antioxidant activity of NCBRPE, demonstrating that it retained most of the extract's original antioxidant capacity. This was further supported by oxidative burst assays, which showed a significant reduction in reactive oxygen species (ROS) production in neutrophils. These findings position NCBRPE as a multi-functional therapeutic candidate for managing infections, oxidative stress, and tumor progression in the ovarian cancer microenvironment. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/13465-5 - Desenvolvimento de nanopartículas poliméricas contendo própolis vermelha brasileira e avaliação antitumoral in vitro e in vivo em cânceres urológicos |
| Beneficiário: | Priscyla Daniely Marcato Gaspari |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar |
| Beneficiário: | Maria Vitória Lopes Badra Bentley |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/01655-7 - Resistoma, plasmidoma e viruloma de Enterobacterales isoladas do meio ambiente carreando genes mcr-like |
| Beneficiário: | Eliana Guedes Stehling |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |