Color Change and Cytotoxic Effects of a Bleaching Gel With 22% Carbamide Peroxide Catalyzed by Manganese Oxide

Autor(es)

Ribeiro, Rafael Antonio de Oliveira ; Peruchi, Victoria ; Sonego, Leonardo Novaes Gaeta ; Soares, Igor Paulino Mendes ; Soares, Diana Gabriela ; Hebling, Josimeri ; Costa, Carlos Alberto de Souza

• Tipo de documento: Artigo Científico
• Fonte: Journal of Esthetic and Restorative Dentistry; v. N/A, p. 9-pg., 2025-01-04.

Resumo

Objectives: To evaluate the color change and trans-amelodentinal cytotoxicity of a 22% carbamide peroxide (CP) bleaching gel containing different concentration of manganese oxide (MnO2). Material and Methods: Enamel/dentin discs adapted to artificial pulp chambers were distributed according to treatments: CN-No treatment; CP22%-22%CP; CP22 + 2MnO(2)-22%CP + 2 mg/mLMnO(2); CP22% + 6MnO(2)-22%CP + 6 mg/mLMnO(2); CP22% + 10MnO(2)-22%CP + 10 mg/mLMnO(2) applied for 2 h for 15 days. Color change-CC (Delta E-00 and Delta WID) (n = 8) was determined at 5, 10, and 15-day periods (ANOVA/Sidak). Trans-amelodentinal cytotoxicity (TC) was assessed after applying the extracts to cultured MDPC-23 cells, which were analyzed concerning their viability (Vi) (n = 8) and oxidative stress (OxS) (n = 8). The amount of hydroxyl radical (OH center dot) (n = 4) generated by H2O2 degradation (ANOVA/Sidak), and trans-amelodentinal diffusion of H2O2 (n = 8) (ANOVA/Tukey) were also evaluated. Results: CP22 + 10MnO(2) presented higher CC than CP22 for both evaluated equations in all analysis periods (p <= 0.04). Among bleached groups, CP22 + 10MnO(2) presented the highest OH center dot generation (p <= 0.02) and the lowest trans-amelodentinal diffusion of H2O2 (p <= 0.0004). Therefore, MDPC-23 cells in CP22 + 10MnO(2) exhibited the lowest OxS (p < 0.0001) and consequently the highest Vi (p <= 0.0146). Conclusion: Besides increasing the bleaching efficacy, the strategy of adding 10 mg/mL of MnO2 to a 22%CP bleaching gel also reduces significantly the trans-amelodentinal diffusion of H2O2, diminishing the cytotoxicity caused by at-home bleaching therapy. (AU)

• Processo FAPESP: 23/02780-5 - Desenvolvimento e aplicação de um primer polimérico contendo TiF4 para clareamento dental de consultório associado a irradiação com LED violeta
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/01184-4 - Desenvolvimento de estratégias inovadoras para clareamento dental de consultório: eficácia estética e biocompatibilidade associadas à redução do tempo clínico
• Beneficiário: Rafael Antonio de Oliveira Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado