Can pigments of different natures interfere with the cytotoxicity from in-office bleaching?

Autor(es)

Ribeiro, Rafael Antonio de Oliveira ; Martins, Beatriz Voss ; Dias, Marlon Ferreira ; Peruchi, Victoria ; Anselmi, Caroline ; Soares, Igor Paulino Mendes ; Hebling, Josimeri ; Cavalli, Vanessa ; Costa, Carlos Alberto de Souza

• Tipo de documento: Artigo Científico
• Fonte: ODONTOLOGY; v. N/A, p. 9-pg., 2025-02-13.

Resumo

Objective To assess the influence of tooth stained with different colored solutions on the cytotoxicity of a 35% H2O2-bleaching gel. Materials and methods The established groups were (n = 8): NC- without treatment (negative control); PC- 35% H2O2 (positive control); CO- coffee + 35% H2O2; BT- black tea + 35% H2O2; WI- wine + 35% H2O2; COBTWI- coffee/black tea/wine + 35% H2O2. Standardized enamel/dentin disks were submitted to staining protocols, adapted to artificial pulp chambers, and bleaching was performed. The chromatic alterations (Delta E-00,E- Delta WID) were assessed after bleaching and the extracts (culture medium + diffused gel components) were collected and applied to odontoblast-like cells. The amount of diffused H2O2, cell viability, and oxidative stress were evaluated and data were submitted to ANOVA/Tukey; p < 0.05). Results Thirty five% H2O2 effectively bleached the stained specimens, regardless of the staining solution used (CO, BT, WI, and COBTWI), and no differences were found among the stained protocols (p > 0.05). All staining protocols reduced H2O2 trans-amelodentinal diffusion (p > 0.05), decreasing bleaching cytotoxicity in comparison to PC (p < 0.05). Conclusion Irrespective of the nature of the pigment used for staining enamel and dentin, the gel with 35% H2O2 effectively bleached the tooth structure. The presence of pigments in both dental tissues, regardless their nature, reduced the trans-amelodentinal diffusion of H2O2 and consequently the cytotoxicity of the bleaching gel. (AU)

• Processo FAPESP: 23/02780-5 - Desenvolvimento e aplicação de um primer polimérico contendo TiF4 para clareamento dental de consultório associado a irradiação com LED violeta
• Beneficiário: Carlos Alberto de Souza Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/01184-4 - Desenvolvimento de estratégias inovadoras para clareamento dental de consultório: eficácia estética e biocompatibilidade associadas à redução do tempo clínico
• Beneficiário: Rafael Antonio de Oliveira Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado