| Texto completo | |
| Autor(es): Mostrar menos - |
Pena, Mauricio S.
;
Tang, Fenny Hui Fen
;
Franco, Fernando Alves de Lima
;
Rodrigues, Alessandro Taunay
;
Carrara, Guilherme Moreira Paiva
;
Araujo, Thais Larissa Silva
;
Giordano, Ricardo Jose
;
Palmisano, Giuseppe
;
de Camargo, Maristela Martins
;
Uliana, Silvia Reni Bortolin
;
Stolf, Beatriz Simonsen
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Parasitology; v. 150, n. 10, p. 12-pg., 2023-09-01. |
| Resumo | |
Leishmaniases affect 12 million people worldwide. They are caused by Leishmania spp., protozoan parasites transmitted to mammals by female phlebotomine flies. During the life cycle, promastigote forms of the parasite live in the gut of infected sandflies and convert into amastigotes inside the vertebrate macrophages. The parasite evades macrophage's microbicidal responses due to virulence factors that affect parasite phagocytosis, survival and/or proliferation. The interaction between Leishmania and macrophage molecules is essential to phagocytosis and parasite survival. Proteins containing leucine-rich repeats (LRRs) are common in several organisms, and these motifs are usually involved in protein-protein interactions. We have identified the LRR17 gene, which encodes a protein with 6 LRR domains, in the genomes of several Leishmania species. We show here that promastigotes of Leishmania (L.) amazonensis overexpressing LaLRR17 are more infective in vitro. We produced recombinant LaLRR17 protein and identified macrophage 78 kDa glucose-regulated protein (GRP78) as a ligand for LaLRR17 employing affinity chromatography followed by mass spectrometry. We showed that GRP78 binds to LaLRR17 and that its blocking precludes the increase of infection conferred by LaLRR17. Our results are the first to report LRR17 gene and protein, and we hope they stimulate further studies on how this protein increases phagocytosis of Leishmania. (AU) | |
| Processo FAPESP: | 14/26777-4 - Papel de cTXNPx e OPB na virulência e resistência a fármacos em Leishmania L. amazonensis e busca de novos fatores de virulência por proteoma comparativo |
| Beneficiário: | Beatriz Simonsen Stolf |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 12/19135-0 - Estudo dos efeitos da proteína LaLRR17 de Leishmania (L.) amazonensis na infecção do macrófago e identificação de seus possíveis ligantes |
| Beneficiário: | Mauricio Scavassini Peña |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/14972-8 - Papel de sCD100 na infecção in vitro e in vivo por diferentes espécies de Leishmania |
| Beneficiário: | Beatriz Simonsen Stolf |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |