| Texto completo | |
| Autor(es): |
de Oliveira, Aline Pereira
;
Naozuka, Juliana
;
Landero-Figueroa, Julio Alberto
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Food Composition and Analysis; v. 142, p. 10-pg., 2025-06-01. |
| Resumo | |
Selenium plays a crucial role in human health, with Se organic species being safer and more bioavailable than inorganic forms. This study aimed to develop a fast and reliable analytical method for Se speciation to evaluate the effectiveness of Pleurotus mushroom enrichment strategies. A reversed-phase cation-exchange bimodal mixed-mode high-performance liquid chromatography - inductively coupled plasma mass spectrometry (RP/CEX HPLC-ICP-MS) method was optimized for the separation and quantification of SeMet, SeCys2, MSC, Se(IV), and Se (VI). Enzymatic hydrolysis was performed using ultrasound-assisted extraction with pepsin and protease XIV in Tris-HCl, achieving high extraction efficiencies (67-102 %). Chromatographic separation was completed within 6.5 minutes under isocratic conditions. The method was applied to Se-enriched Pleurotus mushrooms, revealing effective bioconversion of inorganic Se into SeMet. However, mushrooms grown in Se(VI)-supplemented substrates retained higher levels of inorganic Se, raising potential safety concerns. These findings support the use of Se(IV) as a preferable supplementation strategy for improving the nutritional quality of edible mushrooms. (AU) | |
| Processo FAPESP: | 17/05009-7 - Avaliação do antagonismo entre selênio e elementos tóxicos em cogumelos: enriquecimento e remediação |
| Beneficiário: | Aline Pereira de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/06332-9 - Enriquecimento elementar: essencialidade e toxicidade |
| Beneficiário: | Juliana Naozuka |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 19/00663-6 - O papel da especiação de selênio no antagonismo de selênio e metais tóxicos em cogumelos enriquecidos com selênio |
| Beneficiário: | Aline Pereira de Oliveira |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |