| Texto completo | |
| Autor(es): Mostrar menos - |
Cunha, Camila Venturini Ayres
;
Oharomari, Leandro Kansuke
;
de Lima, Julia Boro
;
de Paula, Leticia Maria
;
Katashima, Carlos Kiyoshi
;
Rios, Thaiane
;
Meneguelli, Talitha
;
Pinto, Ana Paula
;
Buoro, Danillo Henrique Travensolo
;
Carneiro, Fernanda da Silva
;
Ropelle, Eduardo
;
Pauli, Jose Rodrigo
;
da Silva, Adelino Sanchez Ramos
;
Cintra, Dennys Esper
Número total de Autores: 14
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Food Research International; v. 218, p. 14-pg., 2025-10-01. |
| Resumo | |
Inflammatory proteins present in the context of obesity influence the general permeability of organic biological barriers, including the blood-brain barrier (BBB). Inflammation alters junction proteins, resulting in increased permeability, and may also influence the response pattern of barrier proteins responsible for intracellular nutrient transport. Focusing on the MFSD2a protein (major facilitator superfamily domain containing 2 A), which is present in the BBB, is an important translocator of omega-3 fatty acid (w3) from the periphery to the central nervous system (CNS), this study aimed to assess the impact of obesity induced by a high-fat diet (HF) on the MFSD2a modulation in the hypothalamus of mice. Male Swiss mice were subjected to HF, and after the onset of obesity, the MFSD2a gene expression, protein content, and their distribution through the hypothalamic areas were measured. Also, obese animals were treated with w3-rich oil orally to measure the w3 translocation to the brain. Palmitate or TNF alpha was administered intracerebroventricularly (i.c.v) to test the mechanism enrolled on MFSD2a modulation. Surprisingly, MFSD2a was increased in the hypothalamic MFSD2a induced by HF consumption. The biomolecular mapping showed wide MFSD2a distribution throughout the different hypothalamic nuclei but increased in arcuate and paraventricular nucleus induced by HF-diet. The w3 translocator is modulated over the animal's lifetime under both CT and HF diets. The i.c.v. administration of TNF alpha or palmitic did not alter the translocator, thus, the mechanistic influence of obesity on MFSD2a is still open. The supply of w3-rich oils to obese mice had no impact on their central bioaccessibility. These nutrigenomic approaches revealed that not only the obesogenic process impacts the MFSD2a protein in the hypothalamus but also environmental and life circumstances. (AU) | |
| Processo FAPESP: | 19/13210-0 - Avaliação do mecanismo molecular de ação dos ácidos graxos ômega-3 no desenvolvimento precoce da Doença de Alzheimer associada à obesidade e Diabetes tipo 2 em camundongos: papel do receptor GPR120 |
| Beneficiário: | Dennys Esper Corrêa Cintra |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 23/12208-7 - A influência da obesidade na expressão e função do receptor mfsd2a no hipotálamo de camundongos |
| Beneficiário: | Camila Venturini Ayres Cunha |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 13/07607-8 - CMPO - Centro Multidisciplinar de Pesquisa em Obesidade e Doenças Associadas |
| Beneficiário: | Licio Augusto Velloso |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |