| Texto completo | |
| Autor(es): |
Ferreira, Iasmin Rosanne Silva
;
Justino, Isabela Araujo
;
Martins, Ronaldo Braganca
;
Souza, Maria Vitoria Oliveira
;
de Lima, Thais Melquiades
;
de Freitas Pinheiro, Ana Maria
;
Arruda, Eurico
;
Bastos, Jairo Kenupp
;
Marcato, Priscyla Daniely
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SCIENTIFIC REPORTS; v. 15, n. 1, p. 16-pg., 2025-07-01. |
| Resumo | |
The global COVID-19 pandemic, caused by SARS-CoV-2, continues to pose a significant threat to public health and the economy. SARS-CoV-2 is highly contagious, transmitted primarily through direct contact or inhalation of droplets, and can cause severe respiratory illnesses and other health complications, including post-acute COVID-19 syndrome. This study explored the antiviral potential of Brazilian green propolis, a natural product rich in flavonoids and phenolic compounds encapsulated in a microemulsion, to enhance its stability and antiviral effects. Brazilian green propolis extract was encapsulated in a microemulsion (ME-GP) and characterized using various physicochemical techniques. Furthermore, the antiviral and anti-inflammatory activities of ME-GP was evaluated in vitro and ex-vivo against SARS-CoV-2. For this, cells or tonsils were treated with ME-GP followed by infection with SARS-CoV-2. The microemulsion showed a size of approximately 217 nm, negative zeta potential, high encapsulation efficiency for artepillin C and baccharin (< 99%), and a spherical morphology. The ME-GP formulation was evaluated for antiviral activity against multiple SARS-CoV-2 variants (Wuhan, Gamma, Delta, and Omicron) in Caco-2 cells. The results demonstrated a significant reduction in viral load, particularly for the Wuhan and Delta variants, with up to a 99% reduction in viral load under prophylactic treatment conditions. Time-of-addition assays revealed that ME-GP acts at an early stage in the viral life cycle, likely by interfering with viral entry or immediate post-entry events. Additionally, ME-GP was evaluated in human tonsils, demonstrating an 80% reduction in viral load, suggesting its potential to reduce the transmission and progression of infection. Furthermore, ME-GP exhibited anti-inflammatory activity in human tonsils, significantly decreasing IL-1 beta, IL-6, TNF-alpha, and TNF-beta levels. Thus, this study highlights the promising prophylactic and therapeutic potential of nanoencapsulated green propolis for combating SARS-CoV-2 and its variants, providing a natural adjunct in COVID-19 therapy. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/13465-5 - Desenvolvimento de nanopartículas poliméricas contendo própolis vermelha brasileira e avaliação antitumoral in vitro e in vivo em cânceres urológicos |
| Beneficiário: | Priscyla Daniely Marcato Gaspari |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 14/50928-2 - INCT 2014: Nanotecnologia Farmacêutica: uma abordagem transdisciplinar |
| Beneficiário: | Maria Vitória Lopes Badra Bentley |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |