Prolactin differentially modulates the macrophage activity of lactating rats: possible role of reproductive experience

Autor(es)

Roth Carvalho-Freitas, Maria Isabel ^[1] ; Anselmo-Franci, Janete A. ^[2] ; Maiorka, Paulo Cesar ^[1] ; Palermo-Neto, Joao ^[1] ; Felicio, Luciano F. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Pathol, Sch Vet Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Morphol Stomatol & Physiol, Sch Dent, BR-14049 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF REPRODUCTIVE IMMUNOLOGY; v. 89, n. 1, p. 38-45, APR 2011.
• Citações Web of Science: 11

Resumo

Reproductive experience (i.e., pregnancy and lactation) induces physiological changes in mammals. We recently showed that a previous reproductive experience can modulate the activity of dopaminergic hypothalamic systems while decreasing serum prolactin (PRL) levels and oxidative burst activity in peritoneal macrophages. Dopamine receptor antagonists increase serum PRL levels, and both PRL and dopamine receptors might be involved in the modulation of macrophage activity, providing a means of communication between the nervous and immune systems. The present study evaluated the in vitro effects of PRL and the dopamine receptor 02 antagonist domperidone (DOMP) on the peritoneal activity of macrophages from primiparous and multiparous female rats during lactation. Oxidative bursts and phagocytosis in peritoneal macrophages were evaluated by flow cytometry. Primiparous and multiparous Wistar rats, during the period of lactation (i.e., days 5-7 after parturition) were used. Samples of peritoneal fluid from these rats were first incubated with PRL (10 and 100 nM) for different periods of time. The same procedure was repeated to evaluate the effects of DOMP (10 and 100 nM). Our results showed that macrophages from multiparous rats respond more effectively to in vitro incubation with PRL, especially with regard to oxidative bursts and the percentage of phagocytosis. Additionally, these effects were more pronounced after 30 min of incubation. These data suggest that reproductive experience is associated with a reduction in serum PRL levels, and cells in experienced female animals, including their macrophages, become more sensitive to the effects of PRL (C) 2011 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/04224-0 - Efeitos da administração de interleucina-2 no comportamento maternal e na liberação in vivo de dopamina em núcleo accumbens de ratas
• Beneficiário: Luciano Freitas Felicio
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/61553-3 - Acoes da prolactina e da dopamina na atividade de macrofagos: modulacao pela experiencia reprodutiva em femeas.
• Beneficiário: Maria Isabel Roth de Carvalho Freitas
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 04/14128-0 - Neuroimunomodulação: efeitos do estresse e de citocinas nas relações bidirecionais entre os sistemas nervosos central e imune
• Beneficiário: João Palermo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático