| Texto completo | |
| Autor(es): Mostrar menos - |
Teixeira, Samuel Cota
;
de Souza, Guilherme
;
dos Santos, Natalia Carine Lima
;
de Oliveira, Rafael Martins
;
Silva, Nagela Bernadelli Sousa
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de Lima Junior, Joed Pires
;
Rosini, Alessandra Monteiro
;
Luz, Luana Carvalho
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Martinez, Aryani Felixa Fajardo
;
Almeida, Marcos Paulo Oliveira
;
Faria, Guilherme Vieira
;
Alves, Rosiane Nascimento
;
Gomes, Angelica Oliveira
;
Ambrosio, Maria Anita Lemos Vasconcelos
;
Veneziani, Rodrigo Cassio Sola
;
Bastos, Jairo Kenupp
;
Mineo, Jose Roberto
;
Martins, Carlos Henrique Gomes
;
Ferro, Eloisa Amalia Vieira
;
Barbosa, Bellisa Freitas
Número total de Autores: 20
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MICROORGANISMS; v. 13, n. 8, p. 25-pg., 2025-08-20. |
| Resumo | |
Toxoplasma gondii is a globally distributed protozoan parasite and a major cause of congenital infections, particularly in South America. Current therapies for congenital toxoplasmosis are limited by toxicity, long treatment regimens, and suboptimal efficacy, highlighting the urgent need for safer and more effective alternatives. In this study, we evaluated the antiparasitic effects of crude ethanolic extract of Brazilian Red Propolis (BRP) and its isolated compounds, focusing on 7-O-methylvestitol, in human trophoblast (BeWo) cells and third-trimester placental explants. Both BRP and 7-O-methylvestitol significantly reduced T. gondii adhesion, invasion, and intracellular replication, without compromising host cell viability. Ultrastructural analyses revealed irreversible parasite damage, and cytokine profiling demonstrated immunomodulatory effects, with enhanced production of interleukin (IL)-6, IL-8, and macrophage migration inhibitory factor (MIF) in BeWo cells and downregulation of IL-6, MIF, and tumor Necrosis Factor (TNF) in infected placental villi. Notably, 7-O-methylvestitol reproduced and, in some assays, surpassed the antiparasitic activity of BRP, suggesting it as a key bioactive constituent responsible for the therapeutic potential of the extract. These findings support the identification of 7-O-methylvestitol as a promising lead compound for structure-based drug design and repositioning strategies, advancing the development of novel, safe, and targeted therapies against congenital toxoplasmosis. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |