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| Autor(es): Mostrar menos - |
Rissetti, Rafaela Mastrangelo
;
de Paula, Carolina Lechinski
;
Yamada, Amanda Yaeko
;
Bertani, Amanda Maria de Jesus
;
Costa, Ana Beatriz Nascimento
;
Sacchi, Claudio Tavares
;
Campos, Karoline Rodrigues
;
Guerra, Simony Trevisan
;
Guimaraes, Felipe Freitas
;
Joaquim, Samea Fernandes
;
Langoni, Helio
;
Rall, Vera Lucia Mores
;
Bertolini, Amanda Bezerra
;
Paz, Patrik Junior de Lima
;
Bello, Thais Spessotto
;
Filho, Marcelo Fagali Arabe
;
Panegossi, Leticia Colin
;
Reznik, Alec Utida
;
Ribeiro, Marcio Garcia
;
Camargo, Carlos Henrique
Número total de Autores: 20
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Letters in Applied Microbiology; v. 78, n. 10, p. 8-pg., 2025-10-01. |
| Resumo | |
This study aimed to evaluate the antimicrobial susceptibility and clonal relatedness of Klebsiella pneumoniae isolates recovered from the milk of cows with mastitis in a large-scale study that clinical severity was scored. A total of 48 K. pneumoniae complex isolates were subjected to in vitro antimicrobial susceptibility tests (AST), PCR for carbapenemase-encoding genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Thirteen isolates, selected by PFGE type and AST results were subjected to whole-genome sequencing (WGS) for the identification of sequence types (STs) and the detection of antimicrobial resistance genes and virulence-encoding genes. A total of 39 different PFGE restriction profiles were identified. Thirteen different STs, including two novel STs, were identified among the 13 sequenced strains. The blaCTX-M-8, qnrE1, aadA2, cmlA4, dfrA15, sul1, tetA, and tetB genes were identified. Two isolates presented the yersiniabactin-encoding gene ybtAEPQSTUX. Klebsiella pneumoniae isolates from the milk of cows with mastitis clinically scored revealed high genetic diversity, according to both PFGE and MLST analysis, as well as harboring resistance genes commonly found in human clinical isolates. (AU) | |
| Processo FAPESP: | 18/21193-5 - EMU concedido no processo 2017/50333-7: sequenciador genético de DNA |
| Beneficiário: | Carlos Henrique Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 17/50333-7 - Plano de desenvolvimento institucional em pesquisa do Instituto Adolfo Lutz (PDIp) |
| Beneficiário: | Carlos Henrique Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Modernização de Institutos Estaduais de Pesquisa |
| Processo FAPESP: | 18/21192-9 - EMU concedido no processo 2017/50333-7: MALDI-TOF |
| Beneficiário: | Carlos Henrique Camargo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Equipamentos Multiusuários |
| Processo FAPESP: | 15/19688-8 - E. coli, Klebsiella pneumoniae e Enterococcus spp: impacto dos fatores de virulência na mastite bovina e reflexos na saúde pública |
| Beneficiário: | Helio Langoni |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |