| Texto completo | |
| Autor(es): |
Oliveira, Felipe A. A.
;
Buri, Marcus V.
;
Rodriguez, Boris L.
;
Costa-da-Silva, Andre L.
;
Araujo, Helena R. C.
;
Capurro, Margareth L.
;
Lu, Stephen
;
Tanaka, Aparecida S.
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 146, p. 9-pg., 2020-03-01. |
| Resumo | |
Recently, a salivary gland transcriptome study demonstrated that the transcripts of a putative cystatin gene (SeqID AAEL013287; Aacystatins) from Aedes aegypti were increased in DENV2-infected mosquitoes and that silencing of the Aacystatin gene resulted in an increase in DENV titres. In this work, Aacystatin was biochemically characterized; the purified recombinant inhibitor was able to inhibit typical cysteine proteases with a Ki in the nM range. Pulldown assays using Aag2 cell extracts identified a cathepsin L-like peptidase (AaCatL) as a possible target of Aacystatin. Purified recombinant AaCatL had an optimal pH of 5.0 and displayed a preference for Leu, Val and Phe residues at P2, which is common for other cathepsin L-like peptidases. Transcription analysis of Aacystatin and AaCatL in the salivary glands and midgut of DENV2-infected mosquitoes revealed a negative correlation between DENV2 titres and levels of the inhibitor and peptidase, suggesting their involvement in DENV2-mosquito interactions. Considering that apoptosis may play an important role during viral infections, the possible involvement of Aacystatin in staurosporine-induced apoptosis in Aag2 cells was investigated; the results showed higher expression of the inhibitor in treated cells; moreover, pre incubation with rAacystatin was able to increase Aag2 cell viability. (C) 2019 Elsevier B.V. All rights reserved. (AU) | |
| Processo FAPESP: | 13/16609-4 - Conversão do sexo do Aedes aegypti por transgenia |
| Beneficiário: | Helena Corrêa de Araújo Gomes |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 12/03657-8 - Inibidores e proteases de ectoparasitas: relação de estrutura-função e identificação do papel dessas moléculas na interação de vetores de doenças e seus agentes etiológicos |
| Beneficiário: | Aparecida Sadae Tanaka |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |