| Texto completo | |
| Autor(es): Mostrar menos - |
Santana, Amanda Paino
;
Cunha, Daniela Moreira
;
Piazza, Rodolfo Debone
;
Wainwright, Mark
;
Feres, Magda
;
Retamal-Valdes, Belen
;
Molon, Rafael Scaf De
;
Garcia, Valdir Gouveia
;
Theodoro, Leticia Helena
;
Assuncao, Wirley Goncalves
;
Avila, Erica Dorigatti De
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 273, p. 10-pg., 2025-10-31. |
| Resumo | |
This in vitro study aimed to determine a safe concentration of butyl toluidine blue (BuTB) and an appropriate pre-irradiation time for antimicrobial photodynamic therapy (aPDT) to reduce polymicrobial oral biofilm without causing cytotoxicity to human cells. Titanium (Ti) discs simulating implant abutment surfaces were inoculated with saliva from patients diagnosed with peri-implantitis to create a clinically relevant polymicrobial biofilm model. Antimicrobial PDT was performed using BuTB at 0.05 mg/mL and two pre-irradiation times (1 and 5 min), followed by diode laser irradiation (InGaAlP, 660 nm, 75 mW, 60 s, 4.5 J). The antimicrobial efficacy of each protocol was evaluated, and cytotoxicity was assessed on keratinocyte (HaCaT) and human gingival fibroblast (HGF) cell lines using both quantitative and qualitative approaches. Significant bacterial reduction was observed for both 1-min (8.91 +/- 0.5 log(1)(0) CFU/mL; p < 0.0001) and 5-min (7.75 +/- 0.6 log(1)(0) CFU/mL; p < 0.0001) pre-irradiation times, compared to untreated Ti controls (12.21 +/- 0.1 log(1)(0) CFU/mL). Although the 5-min protocol showed slightly greater antimicrobial activity (p = 0.0076), the 1-min protocol demonstrated favorable cell viability and mitochondrial activity in both HaCaT and HGF cells. These results suggest that BuTB-mediated aPDT, at a low concentration and short pre-irradiation time, can effectively reduce polymicrobial biofilm while preserving cell viability in the conditions studied. While further studies are needed, these in vitro findings provide a valuable foundation for developing safer and more effective aPDT protocols for potential future clinical applications. (AU) | |
| Processo FAPESP: | 25/07203-1 - Vedação biológica avançada para integração de tecidos moles |
| Beneficiário: | Daniela Moreira Cunha |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 23/15750-7 - Desvendando o potencial terapêutico da Tanshinona-IIa sulfonato na prevenção e tratamento da periodontite experimental em camundongos |
| Beneficiário: | Rafael Scaf de Molon |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/20719-3 - Um novo revestimento antimicrobiano para liberação de droga direcionado a dispositivos implantares percutâneos |
| Beneficiário: | Érica Dorigatti de Avila |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |
| Processo FAPESP: | 21/09434-0 - Um novo revestimento antimicrobiano para liberação de droga direcionado a dispositivos implantares percutâneos |
| Beneficiário: | Érica Dorigatti de Avila |
| Modalidade de apoio: | Bolsas no Brasil - Jovens Pesquisadores |