| Texto completo | |
| Autor(es): |
Roque, Gabriella S. C.
;
Ribeiro, Luiz Gustavo
;
de Souza, Ana Olivia
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CHEMISTRYSELECT; v. 10, n. 48, p. 16-pg., 2025-12-22. |
| Resumo | |
Seven biologically synthesized silver nanoparticles (AgNPs) were produced using extremophilic fungal species from the phylum Ascomycota, including Aspergillus tubingensis, Aspergillus spp., Bionectria ochroleuca, Cladosporium pini-ponderosae, Fusarium proliferatum, Epicoccum nigrum, and Exserohilum rostratum, all of which were isolated from the Brazilian biodiversity. The nanoparticles were designated based on the initials of the respective fungal species (AgNP-AT, AgNP-Asp, AgNP-BO, AgNP-CPP, AgNP-FP, AgNP-EN, and AgNP-ER) and showed predominantly spherical morphology with hydrodynamic diameters consistent with protein capping (DLS), dimensions between 22 and 86 nm (TEM), negative Zeta potential, and FTIR bands indicative of a fungal-derived biomolecular corona. Cytotoxicity was assessed by the MTT assay on MDA-MB-231 triple-negative breast cancer cells (IC50) and normal human fibroblasts (FN1; CC50) at 24, 48, and 72 h, and the selectivity index (SI = CC50/IC50) was calculated. All AgNPs reduced the MDA-MB-231 viability, and AgNP-AT, AgNP-BO, and AgNP-Asp exhibited the highest selectivity toward tumor cells. The AgNP-AT was the most effective and selected to determine its effects on cell-cycle distribution, apoptosis induction, and mutagenicity. Furthermore, AgNP-AT at 0.03 mM did not induce structural chromosomal aberrations in V79-4 cells. These findings indicate that fungal-derived biogenic AgNP-AT is a potent and selective candidate against triple-negative breast cancer cells, without detectable genotoxicity at its effective concentration. (AU) | |
| Processo FAPESP: | 20/04799-7 - Estudo da ação antitumoral de nanopartículas de prata biogênicas |
| Beneficiário: | Gabriella Sales Calaço Roque |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 20/03883-4 - Estudo do efeito de nanopartículas de prata biogênicas em Zebrafish, Tilápia-do-Nilo e Lambari |
| Beneficiário: | Luiz Gustavo Ribeiro |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |