Decreased immunoexpression of survivin could be a potential marker in human non-alcoholic fatty liver disease progression?

Autor(es)

Stefano, Jose T. ^[1] ; de Oliveira, Claudia P. M. S. ^[1] ; Correa-Giannella, Maria L. ^[2] ; Soares, Ibere C. ^[3] ; Kubrusly, Marcia S. ^[1] ; Bellodi-Privato, Marta ^[3] ; de Mello, Evandro S. ^[3] ; de Lima, Vicencia M. R. ^[1] ; Carrilho, Flair J. ^[1] ; Alves, Venancio A. F. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Dept Gastroenterol LIM 07 & 37, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Cellular & Mol Endocrinol LIM 25, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Sch Med, Dept Pathol LIM 14, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIVER INTERNATIONAL; v. 31, n. 3, p. 377-385, MAR 2011.
• Citações Web of Science: 9

Resumo

Background/aim Regulation of apoptosis in non-alcoholic fatty liver disease (NAFLD) has been a theme of growing debate. Although no other study assessed the role of survivin in NAFLD, its expression has been reported in hepatic carcinogenesis because of other aetiological factors with relevant discrepancies. The aim of this study was to assess the pattern of survivin immunoexpression by tissue microarray along the whole spectrum of NAFLD, including non-alcoholic steatohepatitis (NASH)-related hepatocelular carcinoma (HCC). Methods Liver biopsies from 56 patients with NAFLD were evaluated: 18 with steatosis, 21 non-cirrhotic NASH, 10 NASH-related cirrhosis, seven NASH-related HCC, as compared with 71 HCC related to other causes and with 12 normal livers. Results Survivin immunoexpression in NAFLD was restricted to cytoplasm and was found to be progressively lower in advanced stages, including cirrhosis and HCC: steatosis vs NASH-related cirrhosis (P=0.0243); steatosis vs NASH-related HCC (P=0.0010); NASH vs NASH-related cirrhosis (P=0.0318); and NASH vs NASH-related HCC (P=0.0007), thus suggesting a deregulation of apoptosis from NAFLD towards HCC. Interestingly, survivin immunoreactivity in NASH-related HCC was also found to be significantly lower than in HCC related to other causes (P < 0.05). Remarkably, nuclear staining for survivin was not detected in any case of NAFLD, contrasting to its presence in all other cases of HCC. Conclusions Survivin immunoexpression in NASH-related HCC is herein originally found substantially different than in HCC related to other causes, thus requiring further studies to elucidate the role of survivin in human NAFLD progression. (AU)

• Processo FAPESP: 04/09932-4 - Apoptose em tumores
• Beneficiário: Marcello Fabiano de Franco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 05/55167-0 - Prospeccao funcional de genes diferencialmente expressos na doenca hepatica gordurosa nao alcoolica (dhgna).
• Beneficiário: Jose Tadeu Stefano
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado