A phospholipase A(2) from Bothrops asper snake venom activates neutrophils in culture: Expression of cyclooxygenase-2 and PGE(2) biosynthesis

Autor(es)

Moreira, Vanessa ^[1] ; Gutierrez, Jose Maria ^[2] ; Amaral, Rafaela Bacci ^[1] ; Lomonte, Bruno ^[2] ; Purgatto, Eduardo ^[3] ; Teixeira, Catarina ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Farmacol Lab, BR-05503900 Sao Paulo - Brazil

^[2] Univ Costa Rica, Fac Microbiol, Inst Clodomiro Picado, San Jose - Costa Rica

^[3] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 57, n. 2, p. 288-296, FEB 2011.
• Citações Web of Science: 7

Resumo

In this study, the production of prostaglandin E(2) (PGE(2)) and up-regulation in cyclooxygenase (COX) pathway induced by a phospholipase A(2) (PLA(2)), myotoxin-III (MT-III), purified from Bothrops asper snake venom, in isolated neutrophils were investigated. The arachidonic acid (AA) production and the participation of intracellular PLA(2)s (cytosolic PLA(2) and Ca(2+)-independent PLA(2)) in these events were also evaluated. MT-III induced COX-2, but not COX-1 gene and protein expression in neutrophils and increased PGE(2) levels. Pretreatment of neutrophils with COX-2 and COX-1 inhibitors reduced PGE(2) production induced by MT-III. Arachidonyl trifluoromethyl ketone (AACOCF(3)), an intracellular PLA(2) inhibitor, but not bromoenol lactone (BEL), an iPLA(2) inhibitor, suppressed the MT-III-induced AA and PGE(2) release. In conclusion, MT-III directly stimulates neutrophils inducing COX-2 mRNA and protein expression followed by production of PGE(2). COX-2 isoform is preeminent over COX-1 for production of PGE(2) stimulated by MT-III. PGE(2) and AA release by MT-III probably is related to cPLA(2) activation. (c) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/03336-9 - Estudo de efeitos de duas fosfolipases A2, isoladas do veneno de serpente, sobre vias de ativação do NF-kB relacionados à expressão de COX-2 e de PGE sintase M-1: envolvimento do receptor de manose
• Beneficiário: Catarina de Fatima Pereira Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/03337-5 - Efeito de duas fosfolipases A2, isoladas do veneno de serpente, sobre vias de ativação do NF-kapa b relacionadas à expressão de COX-2 e de PGE sintase m-1: envolvimento do receptor de manose de macrófagos nesses eventos
• Beneficiário: Vanessa Moreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado