| Texto completo | |
| Autor(es): |
Costa-Silva, Tales A.
[1]
;
Nogueira, Marco A.
[1]
;
Fernandes Souza, Claudia R.
[1]
;
Oliveira, Wanderley P.
[1]
;
Said, Suraia
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Drying Technology; v. 29, n. 9, p. 1112-1119, 2011. |
| Citações Web of Science: | 17 |
| Resumo | |
The present work deals with improving the production and stabilization of lipases from Cercospora kikuchii. Maximum enzyme production (9.384 U/ml) was obtained after 6 days in a medium supplemented with 2% soybean oil. The lipases were spray dried with different adjuvants, and their stability was studied. The residual enzyme activity after drying with 10% (w/v) of lactose, b- cyclodextrin, maltodextrin, mannitol, gum arabic, and trehalose ranged from 63 to 100%. The enzyme activity was lost in the absence of adjuvants. Most of the adjuvants used kept up at least 50% of the enzymatic activity at 5 degrees C and 40% at 25 degrees C after 8 months. The lipase dried with 10% of beta-cyclodextrin retained 72% of activity at 5 degrees C. Lipases were separated by butyl-sepharose column into 4 pools, and pool 4 was partially purified (33.1%; 269.5 U/mg protein). This pool was also spray dried in maltodextrin DE10, and it maintained 100% of activity. (AU) | |
| Processo FAPESP: | 06/50955-3 - Seleção de fungos de solo e endofíticos produtores de lipases e adaptação das condições de cultivo |
| Beneficiário: | Marco Antonio Nogueira |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 04/07935-6 - Bioprospecção de fungos: a busca de compostos importantes para o projeto de remédios e enzimas para aplicações farmacêuticas e industriais |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |
| Processo FAPESP: | 08/52732-7 - Caracterização bioquímica e secagem por spray-dryer de lipases produzidas pelo fungo endofítico Cercospora kikuchii |
| Beneficiário: | Tales Alexandre da Costa e Silva |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |