| Full text | |
| Author(s): |
Costa-Silva, Tales A.
[1]
;
Nogueira, Marco A.
[1]
;
Fernandes Souza, Claudia R.
[1]
;
Oliveira, Wanderley P.
[1]
;
Said, Suraia
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
Total Affiliations: 1
|
| Document type: | Journal article |
| Source: | Drying Technology; v. 29, n. 9, p. 1112-1119, 2011. |
| Web of Science Citations: | 17 |
| Abstract | |
The present work deals with improving the production and stabilization of lipases from Cercospora kikuchii. Maximum enzyme production (9.384 U/ml) was obtained after 6 days in a medium supplemented with 2% soybean oil. The lipases were spray dried with different adjuvants, and their stability was studied. The residual enzyme activity after drying with 10% (w/v) of lactose, b- cyclodextrin, maltodextrin, mannitol, gum arabic, and trehalose ranged from 63 to 100%. The enzyme activity was lost in the absence of adjuvants. Most of the adjuvants used kept up at least 50% of the enzymatic activity at 5 degrees C and 40% at 25 degrees C after 8 months. The lipase dried with 10% of beta-cyclodextrin retained 72% of activity at 5 degrees C. Lipases were separated by butyl-sepharose column into 4 pools, and pool 4 was partially purified (33.1%; 269.5 U/mg protein). This pool was also spray dried in maltodextrin DE10, and it maintained 100% of activity. (AU) | |
| FAPESP's process: | 06/50955-3 - Selection of soil fungi and lipase producing endophytics and adaptation of the cultivation conditions |
| Grantee: | Marco Antonio Nogueira |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 04/07935-6 - Bioprospection in fungi: the search of lead compounds for drug design and enzymes for pharmaceutical and industrial applications |
| Grantee: | Jairo Kenupp Bastos |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 08/52732-7 - Biochemical characterization and spray drying of lipases produced by the endophytic fungus Cercospora kicuchii |
| Grantee: | Tales Alexandre da Costa e Silva |
| Support Opportunities: | Scholarships in Brazil - Master |