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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis

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Autor(es):
Sassaki, C. Y. [1] ; Colodel, M. M. [1] ; Ferreira, I [1] ; Nogueira, F. S. [2] ; Lucheis, S. B. [3] ; Langoni, H. [4] ; Rocha, N. S. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] UNESP Univ Estadual Paulista, Dept Clin Vet Med, Sch Vet Med & Anim Husb, Sao Paulo State Univ, Botucatu, SP - Brazil
[2] Educ Fdn Andradina FEA, Sch Vet Med, Andradina, SP - Brazil
[3] Sao Paulo Agcy Agribusiness Technol APTA, Bauru, SP - Brazil
[4] UNESP Univ Estadual Paulista, Dept Vet Hyg & Publ Hlth, Sch Vet Med & Anim Husb, Sao Paulo State Univ, Botucatu, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 17, n. 3, p. 348-352, 2011.
Citações Web of Science: 0
Resumo

Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node aspirate culture and PCR of hemoculture. All infected and uninfected animals had positive and negative results in at least one test. In conclusion, the association of several tests improves the efficacy of canine visceral leishmaniasis diagnosis. (AU)

Processo FAPESP: 05/56765-9 - Comparacao dos resultados da hemocultura, citologia aspirativa de linfonodos, reacao de imunofluorescencia indireta(rifi) e pcr no diagnostico de leishmaniose canina em animais vacinados e nao ...
Beneficiário:Carla Yumi Sassaki
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica