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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison of different diagnostic tests in dogs uninfected and naturally infected with visceral leishmaniasis

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Author(s):
Sassaki, C. Y. [1] ; Colodel, M. M. [1] ; Ferreira, I [1] ; Nogueira, F. S. [2] ; Lucheis, S. B. [3] ; Langoni, H. [4] ; Rocha, N. S. [1]
Total Authors: 7
Affiliation:
[1] UNESP Univ Estadual Paulista, Dept Clin Vet Med, Sch Vet Med & Anim Husb, Sao Paulo State Univ, Botucatu, SP - Brazil
[2] Educ Fdn Andradina FEA, Sch Vet Med, Andradina, SP - Brazil
[3] Sao Paulo Agcy Agribusiness Technol APTA, Bauru, SP - Brazil
[4] UNESP Univ Estadual Paulista, Dept Vet Hyg & Publ Hlth, Sch Vet Med & Anim Husb, Sao Paulo State Univ, Botucatu, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 17, n. 3, p. 348-352, 2011.
Web of Science Citations: 0
Abstract

Uninfected dogs (n = 10) and those naturally infected with leishmaniasis (n = 10) were subjected to several diagnostic tests, namely: hemoculture, polymerase chain reaction (PCR) of hemoculture, indirect immunofluorescence (RIFI), cytological examination of lymph node aspirate, culture of lymph node aspirate and PCR of lymph node aspirate. RIFI - followed by PCR of lymph node aspirate culture - presented more positive results in infected dogs than in uninfected ones. In infected animals, RIFI was more effective than PCR of lymph node aspirate culture. There was no statistical difference in positivity between RIFI and hemoculture; lymph node aspirate culture/cytological examination of lymph node aspirate and PCR of hemoculture; and between PCR of lymph node aspirate culture and PCR of hemoculture. All infected and uninfected animals had positive and negative results in at least one test. In conclusion, the association of several tests improves the efficacy of canine visceral leishmaniasis diagnosis. (AU)