Cloning and Identification of a Complete cDNA Coding for a Bactericidal and Antitumoral Acidic Phospholipase A(2) from Bothrops jararacussu Venom

Autor(es)

Roberto, Patricia G. ^[1] ; Kashima, Simone ^[1] ; Marcussi, Silvana ^[1] ; Pereira, Jose O. ^[2] ; Astolfi-Filho, Spartaco ^[2] ; Nomizo, Auro ^[3] ; Giglio, Jose R. ^[4] ; Fontes, Marcos R. M. ^[5] ; Soares, Andreimar M. ^[1] ; Franca, Suzelei C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Ribeirao Preto, UNAERP, Unidade Biotecnol, Ribeirao Preto, SP - Brazil

^[2] Univ Fed Amazonas, Fac Ciencias Agr, Manaus, Amazonas - Brazil

^[3] Univ Sao Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, FMRP, Dept Bioquim & Imunol, BR-14049 Ribeirao Preto, SP - Brazil

^[5] Univ Estadual Paulista, IB, Dept Fis & Biofis, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: The Protein Journal; v. 23, n. 4, p. 273-285, May 2004.
• Área do conhecimento: Ciências Biológicas - Farmacologia
• Citações Web of Science: 46
• Assunto(s): Venenos de origem animal Bothrops Jararacuçu Fosfolipases

Resumo

In order to better understand the function of acidic phospholipases A2 (PLA2s) from snake venoms, expressed sequence tags (ESTs) that code for acidic PLA2s were isolated from a cDNA library prepared from the poly(A)+ RNA of venomous glands of Bothrops jararacussu. The complete nucleotide sequence (366 bp), named BOJU-III, encodes the BthA-I-PLA2 precursor, which includes a signal peptide and the mature protein with 16 and 122 amino acid residues, respectively. Multiple comparison of both the nucleotide and respective deduced amino acid sequence with EST and protein sequences from databases revealed that the full-length cDNA identified (BOJU III - AY145836) is related to an acidic PLA2 sharing similarity, within the range 55-81%, with acidic phospholipases from snake venoms...(AU)

• Processo FAPESP: 04/00464-8 - Caracterizacao funcional e estrutural de uma metaloprotease com atividades bactericida e anti-tumoral isolada do veneno de bothrops jararacussu.
• Beneficiário: Silvana Marcussi
• Modalidade de apoio: Bolsas no Brasil - Doutorado