| Full text | |
| Author(s): |
Roberto, Patricia G.
[1]
;
Kashima, Simone
[1]
;
Marcussi, Silvana
[1]
;
Pereira, Jose O.
[2]
;
Astolfi-Filho, Spartaco
[2]
;
Nomizo, Auro
[3]
;
Giglio, Jose R.
[4]
;
Fontes, Marcos R. M.
[5]
;
Soares, Andreimar M.
[1]
;
Franca, Suzelei C.
[1]
Total Authors: 10
|
| Affiliation: | [1] Univ Ribeirao Preto, UNAERP, Unidade Biotecnol, Ribeirao Preto, SP - Brazil
[2] Univ Fed Amazonas, Fac Ciencias Agr, Manaus, Amazonas - Brazil
[3] Univ Sao Paulo, FCFRP, Dept Anal Clin Toxicol & Bromatol, BR-14049 Ribeirao Preto, SP - Brazil
[4] Univ Sao Paulo, FMRP, Dept Bioquim & Imunol, BR-14049 Ribeirao Preto, SP - Brazil
[5] Univ Estadual Paulista, IB, Dept Fis & Biofis, Botucatu, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | The Protein Journal; v. 23, n. 4, p. 273-285, May 2004. |
| Field of knowledge: | Biological Sciences - Pharmacology |
| Web of Science Citations: | 46 |
| Abstract | |
In order to better understand the function of acidic phospholipases A2 (PLA2s) from snake venoms, expressed sequence tags (ESTs) that code for acidic PLA2s were isolated from a cDNA library prepared from the poly(A)+ RNA of venomous glands of Bothrops jararacussu. The complete nucleotide sequence (366 bp), named BOJU-III, encodes the BthA-I-PLA2 precursor, which includes a signal peptide and the mature protein with 16 and 122 amino acid residues, respectively. Multiple comparison of both the nucleotide and respective deduced amino acid sequence with EST and protein sequences from databases revealed that the full-length cDNA identified (BOJU III - AY145836) is related to an acidic PLA2 sharing similarity, within the range 55-81%, with acidic phospholipases from snake venoms...(AU) | |