| Texto completo | |
| Autor(es): |
Castro, Leandro M.
[1, 2]
;
Berti, Denise A.
[2]
;
Russo, Lilian C.
[2]
;
Coelho, Veronica
[3, 4]
;
Gozzo, Fabio C.
[5]
;
Oliveira, Vitor
[1]
;
Ferro, Emer S.
[2]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Biochem, BR-04044020 Sao Paulo - Brazil
[2] Inst Biomed Sci, Dept Dev & Cell Biol, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Immunol Lab, Heart Inst InCor, Sch Med, BR-05403001 Sao Paulo - Brazil
[4] Natl Inst Sci & Technol, INCT Inst Invest Immunol 3, BR-05403001 Sao Paulo - Brazil
[5] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AAPS JOURNAL; v. 12, n. 4, p. 608-616, DEC 2010. |
| Citações Web of Science: | 11 |
| Resumo | |
Cells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role. (AU) | |
| Processo FAPESP: | 10/00828-0 - Estudos funcionais sobre oligopeptídeos e oligopeptidases intracelulares e suas possíveis correlações fisiopatológicas |
| Beneficiário: | Lilian Cristina Russo Vieira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 04/04933-2 - Biologia celular molecular de oligopeptidases |
| Beneficiário: | Emer Suavinho Ferro |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |