Similar Intracellular Peptide Profile of TAP1/beta 2 Microglobulin Double-Knockout Mice and C57BL/6 Wild-Type Mice as Revealed by Peptidomic Analysis

Author(s)

Castro, Leandro M. ^{[1, 2]} ; Berti, Denise A. ^[2] ; Russo, Lilian C. ^[2] ; Coelho, Veronica ^{[3, 4]} ; Gozzo, Fabio C. ^[5] ; Oliveira, Vitor ^[1] ; Ferro, Emer S. ^[2]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Biochem, BR-04044020 Sao Paulo - Brazil

^[2] Inst Biomed Sci, Dept Dev & Cell Biol, BR-05508000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Immunol Lab, Heart Inst InCor, Sch Med, BR-05403001 Sao Paulo - Brazil

^[4] Natl Inst Sci & Technol, INCT Inst Invest Immunol 3, BR-05403001 Sao Paulo - Brazil

^[5] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil

• Document type: Journal article
• Source: AAPS JOURNAL; v. 12, n. 4, p. 608-616, DEC 2010.
• Web of Science Citations: 11

Abstract

Cells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role. (AU)

• FAPESP's process: 10/00828-0 - Functional studies about intracellular oligopeptides and oligopeptidases and their possible physiopatological correlations
• Grantee: Lilian Cristina Russo Vieira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 04/04933-2 - Molecular cell biology of oligopeptidases
• Grantee: Emer Suavinho Ferro
• Support Opportunities: Research Projects - Thematic Grants