| Full text | |
| Author(s): |
Castro, Leandro M.
[1, 2]
;
Berti, Denise A.
[2]
;
Russo, Lilian C.
[2]
;
Coelho, Veronica
[3, 4]
;
Gozzo, Fabio C.
[5]
;
Oliveira, Vitor
[1]
;
Ferro, Emer S.
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biochem, BR-04044020 Sao Paulo - Brazil
[2] Inst Biomed Sci, Dept Dev & Cell Biol, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Immunol Lab, Heart Inst InCor, Sch Med, BR-05403001 Sao Paulo - Brazil
[4] Natl Inst Sci & Technol, INCT Inst Invest Immunol 3, BR-05403001 Sao Paulo - Brazil
[5] Univ Estadual Campinas, Inst Chem, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | AAPS JOURNAL; v. 12, n. 4, p. 608-616, DEC 2010. |
| Web of Science Citations: | 11 |
| Abstract | |
Cells produce and use peptides in distinctive ways. In the present report, using isotope labeling plus semi-quantitative mass spectrometry, we evaluated the intracellular peptide profile of TAP1/beta 2m(-/-) (transporter associated with antigen-processing 1/beta 2 microglobulin) double-knockout mice and compared it with that of C57BL/6 wild-type animals. Overall, 92 distinctive peptides were identified, and most were shown to have a similar concentration in both mouse strains. However, some peptides showed a modest increase or decrease (similar to 2-fold), whereas a glycine-rich peptide derived from the C-terminal of neurogranin (KGPGPGGPGGAGGARGGAGGGPSGD) showed a substantial increase (6-fold) in TAP1/beta 2m(-/-) mice. Thus, TAP1 and beta 2microglobulin have a small influence on the peptide profile of neuronal tissue, suggesting that the presence of peptides derived from intracellular proteins in neuronal tissue is not associated with antigens of the class I major histocompatibility complex. Therefore, it is possible that these intracellular peptides play a physiological role. (AU) | |
| FAPESP's process: | 10/00828-0 - Functional studies about intracellular oligopeptides and oligopeptidases and their possible physiopatological correlations |
| Grantee: | Lilian Cristina Russo Vieira |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 04/04933-2 - Molecular cell biology of oligopeptidases |
| Grantee: | Emer Suavinho Ferro |
| Support Opportunities: | Research Projects - Thematic Grants |