Molecular characterization of G and P-types bovine rotavirus strains from Goiás, Brazil: high frequency of mixed P-type infections

Autor(es)

Ramos Caruzo, Thabata Alessandra ^[1] ; Elsner Diederichsen de Brito, Willia Marta ^[2] ; Munford, Veridiana ^[3] ; Racz, Maria Lucia ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolucao & Bioagentes, Campinas, SP - Brazil

^[2] Univ Fed Goias, Lab Virol Anim, Inst Patol Trop & Saude Publ, Goiania, Go - Brazil

^[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 8, p. 1040-1043, DEC 2010.
• Citações Web of Science: 13

Resumo

In this study, 331 samples from calves less than one month old from a dairy herd in the district of Piracanjuba, state of Goiás, Brazil were tested for rotavirus. Thirty-three samples (9.9%) tested positive for rotavirus. Out of those, 31 were submitted to G and P characterization by reverse transcription followed by semi-nested polymerase chain reaction. Two samples were characterized as G6P[1], three as G10P[11] and five as G6P[11]. The majority of the samples (51.6%) displayed multiple P genotypes (P-genotype mixtures), including typical human genotypes P[4] and P[6M], suggesting the occurrence of co-infections and genetic reassortment. Also, the detection of human genotypes in bovine samples may be considered evidence of the zoonotic potential of rotaviruses. To our knowledge, this is the first report of such a high frequency of P genotype mixtures in bovine rotavirus samples. It also increases data on G and P rotavirus genotypes circulating in dairy herds in Brazil and can help in the development of more efficient immunization approaches, thereby controlling infection and reducing economical losses. (AU)

• Processo FAPESP: 99/04575-9 - Caracterizacao sorologica e molecular de rotavirus de humanos e de animais.
• Beneficiário: Maria Lucia Barbosa de Oliveira Racz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 93/01848-8 - Identificação e cultivo de Rotavírus de animais em culturas celulares
• Beneficiário: Maria Lucia Barbosa de Oliveira Racz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 01/14336-3 - Sequenciamento genômico de amostras atípicas de rotavírus bovino do estado de Goiás
• Beneficiário: Thabata Alessandra Ramos Caruzo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto