Comparative analysis of newborn and adult Bothrops jararaca snake venoms

Autor(es)

Antunes, Thatiane C. ^[1] ; Yamashita, Karine M. ^[1] ; Barbaro, Katia C. ^[2] ; Saiki, Mitiko ^[3] ; Santoro, Marcelo L. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Lab Physiopathol, BR-05503900 Sao Paulo - Brazil

^[2] Inst Butantan, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil

^[3] IPEN CNEN SP, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 56, n. 8, p. 1443-1458, DEC 2010.
• Citações Web of Science: 53

Resumo

Different clinical manifestations have been reported to occur in patients bitten by newborn and adult Bothrops jararaca snakes. Herein, we studied the chemical composition and biological activities of B. jararaca venoms and their immunoneutralization by commercial antivenin at these ontogenetic stages. Important differences in protein profiles were noticed both in SDS-PAGE and two-dimensional electrophoresis. Newborn venom showed lower proteolytic activity on collagen and fibrinogen, diminished hemorrhagic activity in mouse skin and hind paws, and lower edematogenic, ADPase and 5'-nucleotidase activities. However, newborn snake venom showed higher L-amino oxidase, hyaluronidase, platelet aggregating, procoagulant and protein C activating activities. The adult venom is more lethal to mice than the newborn venom. In vitro and in vivo immunoneutralization tests showed that commercial Bothrops sp antivenin is less effective at neutralizing newborn venoms. These findings indicate remarkable differences in biological activities of B. jararaca venom over its development. We suggest that not only venom from adult specimens, but also from specimens at other ontogenetic stages should be included in the venom pool used for raising antibodies. Thus, Bothrops antivenin can efficaciously neutralize proteins lacking in the adult venom pool, especially those that promote more intense hemostatic disturbances in victims of newborn snakes. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/00266-2 - Possíveis divergências entre as atividades biológicas dos venenos de serpentes Bothrops jararaca adultas e recém-nascidas
• Beneficiário: Thatiane Cristina Antunes
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 08/02996-8 - Venenos de serpentes Bothrops jararaca recém-nascidas e adultas: comparação entre suas atividades enzimáticas e biológicas, imunoneutralização, composição química e capacidade de induzir alterações hemostáticas
• Beneficiário: Marcelo Larami Santoro
• Modalidade de apoio: Auxílio à Pesquisa - Regular