Evidence that metyrapone in the presence of inflammation modulates cytokine mRNA expression

Autor(es)

Peruzzo, Daiane C. ^[1] ; Benatti, Bruno B. ; Andersen, Monica L. ^[2] ; Tufik, Sergio ^[2] ; Casati, Marcio Z. ; Nociti, Jr., Francisco H.

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Fac Odontol Piracicaba, Div Periodont, Sch Dent Piracicaba, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 52, n. 3, p. 184-189, DEC 2010.
• Citações Web of Science: 2

Resumo

Objective: Metyrapone (MT) has been used clinically to decrease glucocorticoid levels in human and animal studies. However, the potential effects of MT in the presence of inflammation are poorly understood. Thus, the aim of this study was to evaluate the effects of the administration of MT on the mRNA levels of pro-inflammatory cytokines in the presence of inflammation induced by the well-established model of ligature-induced periodontitis in rats. Material and methods: Sixty animals were randomly assigned into three experimental groups of 20 rats each: G1-control; G2-periodontal disease (PD) induced by cotton ligature; G3-PD associated with 3 daily doses of MT (50 mg/kg/3 x 3 h). After 30 days, all animals were killed by decapitation. Blood samples were taken and the concentrations of corticosterone and catecholamines measured. Marginal tissues around ligated and non-ligated teeth were harvested and gene expression was assessed by quantitative polymerase chain reaction technique (qPCR). Moreover, the area of interradicular bone loss (ABL) was histometrically determined. Results: Data analysis showed that: (i) ligature placement resulted in a significant ABL, as compared to non-ligated sites of G1 group; (ii) mRNA levels of all the pro-inflammatory factors assessed (INF-gamma, TNF-alpha, IL-1 beta and IL-6) were increased in the PD group (G2) (p < 0.05) when compared to G1; (iii) there were no significant differences in corticosterone and catecholamine plasmatic levels between the three groups; (iv) MT administration, in the presence of inflammation, induces an increased ABL and significantly increased mRNA levels of all pro-inflammatory cytokines analyzed (p < 0.05). Conclusion: Within the limits of this study, it can be concluded that MT in the presence of inflammation may modulate expression of pro-inflammatory cytokines, regardless of its effect on plasma corticosterone levels. (C) 2010 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 04/14361-6 - Projeto 1: A influência do estresse crônico na modulação da resposta imune-inflamatória frente a doença periodontal induzida em ratos. Projeto 2: Influência do fator idade na modulação da resposta de células do ligamento periodontal induzida pelo fator de crescimento básico de fibroblastos (bFGF)
• Beneficiário: Francisco Humberto Nociti Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/14363-9 - A influência do estresse crônico na modulação da resposta imune-inflamatória frente à doença periodontal induzida em ratos
• Beneficiário: Daiane Cristina Peruzzo
• Modalidade de apoio: Bolsas no Brasil - Doutorado