Structural and biological characterization of three novel mastoparan peptides from the venom of the neotropical social wasp Protopolybia exigua (Saussure)

Autor(es)

Mendes, Maria Anita ^[1] ; Souza, Bibiana Monson de ; Palma, Mario Sergio

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Instituto de Biociências. Departamento de Biologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 45, n. 1, p. 101-106, Jan. 2005.
• Área do conhecimento: Ciências Biológicas - Bioquímica
• Assunto(s): Peptídeos Venenos de origem animal Vespas Protopolybia exigua

Resumo

The venom of the Neotropical social wasp Protopolybia exigua(Saussure) was fractionated by RP-HPLC resulting in the elution of 20 fractions. The homogeneity of the preparations were checked out by using ESI-MS analysis and the fractions 15, 17 and 19 (eluted at the most hydrophobic conditions) were enough pure to be sequenced by Edman degradation chemistry, resulting in the following sequences: Protopolybia MPI I-N-W-L-K-L-G-K-K-V-S-A-I-L-NH2 Protopolybia-MP II I-N-W-K-A-I-I-E-A-A-K-Q-A-L-NH2 Protopolybia-MP III I-N-W-L-K-L-G-K-A-V-I-D-A-L-NH2. All the peptides were manually synthesized on-solid phase and functionally characterized. Protopolybia-MP I is a hemolytic mastoparan, probably acting on mast cells by assembling in plasma membrane, resulting in pore formation; meanwhile, the peptides Protopolybia-MP II and -MP III were characterized as a non-hemolytic mast cell degranulator toxins, which apparently act by virtue of their binding to G-protein receptor, activating the mast cell degranulation. (AU)

• Processo FAPESP: 01/05060-4 - Caracterizacao estrutural de peptideos biologicamente ativos das peconhas de vespas sociais e solitarias, por espectrometria de massas.
• Beneficiário: Maria Anita Mendes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 03/00985-5 - Estrutura e funcao de mastoparanos dos venenos de vespas.
• Beneficiário: Bibiana Monson de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado