Structural characterization of novel chemotactic and mastoparan peptides from the venom of the social wasp Agelaia pallipes pallipes by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry

Autor(es)

Mendes, Maria Anita ^[1] ; Souza, Bibiana Monson de ; Santos, Lucilene Delazari dos ; Palma, Mario Sergio

Afiliação do(s) autor(es)

^[1] Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Instituto de Biociências. Departamento de Biologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Rapid Communications in Mass Spectrometry; v. 18, n. 6, p. 636-642, Mar. 2004.
• Área do conhecimento: Ciências Biológicas - Bioquímica
• Assunto(s): Peptídeos Espectrometria de massas Venenos de origem animal Vespas

Resumo

High-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) and high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) techniques were applied for the detection, purification, monitoring, and sequencing of two novel and biologically active peptides occurring at very low levels in the venom of the wasp Agelaia pallipes pallipes. These peptides were sequenced under LC/ESI-MS/ MS conditions and designated as Agelaia-CP (I/L-L-G-T-1-L-G-L-L-K-G-I/L-NH2, MW 1207.813a) and Agelaia-MP (I/L-N-W-L-K-L-G-K-A-I-I-D-A-I/L-NH2, MW 1565.013a). The peptide Agelaia-CP showed no hemolytic activity, but it behaved as a mast cell degranulator and induced a potent chemotaxis in polymorphonucleated leukocyte (PMNL) cells, typical of a wasp chemotactic peptide. The peptide Agelaia-MP showed both powerful mast cell degranulation and hemolysis of washed rat red blood cells, and is thus assigned as a new member of the mastoparan family of peptides. Both peptides seem to be directly involved in the strong inflammatory reactions associated with wasp stings. (AU)

• Processo FAPESP: 01/05060-4 - Caracterizacao estrutural de peptideos biologicamente ativos das peconhas de vespas sociais e solitarias, por espectrometria de massas.
• Beneficiário: Maria Anita Mendes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 00/08880-0 - Peptideos policationicos do veneno de "polybia paulista ( hymenoptera,polistinae)" estrutura e funcao.
• Beneficiário: Bibiana Monson de Souza
• Modalidade de apoio: Bolsas no Brasil - Mestrado