Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood

Autor(es):Mostrar menos -

Rigato, Paula Ordonhez ; Maciel, Jr., Milton ^[1] ; Goldoni, Adriana Leticia ; Piubelli, Orlando ; de Brito, Cyro Alves ; Fusaro, Ana Elisa ; Eurico de Alencar, Liciana Xavier ^[2] ; August, Thomas ^[3] ; Azevedo Marques, Jr., Ernesto Torres ^{[2, 4]} ; da Silva Duarte, Alberto Jose ; Sato, Maria Notomi ^[5]

Afiliação do(s) autor(es)

^[1] Univ Maryland, Ctr Vaccine Dev, Baltimore, MD 21201 - USA

^[2] Fiocruz MS, Lab Virol & Terapia Expt, Ctr Pesquisas Aggeu Magalhaes CPqAM, Rio De Janeiro - Brazil

^[3] Johns Hopkins Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD - USA

^[4] Ctr Vaccine Res, Dept Infect Dis & Microbiol, Pittsburg, KS - USA

^[5] Univ Sao Paulo, Lab Dermatol & Imunodeficiencia, Fac Med, Inst Trop Med, Sch Med, LIM 56, BR-05403000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VIROLOGY; v. 406, n. 1, p. 37-47, OCT 10 2010.
• Citações Web of Science: 9

Resumo

Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-gamma, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric IAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 04/14443-2 - Estudo da imunogenicidade materna e neonatal murina a vacina quimérica de DNA que codifica a proteína p55 do HIV-1 direcionada ao compartimento de classe II do complexo principal de histocompatibilidade pela proteína associada à membrana lisossomal 1
• Beneficiário: Maria Notomi Sato
• Modalidade de apoio: Auxílio à Pesquisa - Regular