Protection against Paracoccidioides brasiliensis infection conferred by the prophylactic administration of native and recombinant ArtinM

Autor(es)

Coltri, Kely C. ^[1] ; Oliveira, Leandro L. ^[2] ; Ruas, Luciana P. ^[1] ; Vendruscolo, Patricia E. ^[1] ; Goldman, Maria Helena ^[3] ; Panunto-Castelo, Ademilson ^[4] ; Roque-Barreira, Maria-Cristina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Vicosa, Dept Biol Geral, Vicosa, MG - Brazil

^[3] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Riberirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Dept Enfermagem Geral & Especializada, Escola Enfermagem Ribeirao Preto, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Medical Mycology; v. 48, n. 6, p. 792-799, SEP 2010.
• Citações Web of Science: 18

Resumo

We determined the prophylactic effect of both the d-mannose-binding lectin ArtinM extracted from the seeds of Artocarpus integrifolia (jackfruit) and its recombinant counterpart during the course of experimental paracoccidioidomycosis induced in BALB/c mice. Four experimental protocols of prophylaxis were employed to evaluate the most protective regimen of ArtinM administration. It was demonstrated that the best effect was obtained by administration of two ArtinM doses on days 10 and 3 before the challenge with Paracoccidioides brasiliensis. By following this protocol, the lungs of mice that received native or recombinant ArtinM exhibited reduced fungal burden and granuloma incidence. In addition, the protocol augmented contents of IL-12, IFN-gamma, TNF-alpha and NO. On the other hand, the control group consisting of untreated infected mice had higher pulmonary levels of IL-4 and IL-10. In conclusion, prophylaxis with ArtinM significantly reproduces the effect of its therapeutic administration, i.e, it confers resistance to P. brasiliensis infection in mouse models by promoting IL-12 production and favours Th1-immunity. (AU)

• Processo FAPESP: 05/00303-7 - Regulacao por galectina-3 da producao de il-12 por celulas dentriticas na paracoccidioidomicose experimental.
• Beneficiário: Luciana Pereira Ruas
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 02/12725-5 - Interferência da administração da lectina KM+ no curso da infecção experimental por Paracoccidioides brasiliensis
• Beneficiário: Kely Cristine Coltri
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 06/60642-2 - Efeitos biológicos e aplicações farmacêuticas de lectinas
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 00/09333-2 - Reconhecimento de carboidratos: papéis desempenhados na relação parasita-hospedeiro
• Beneficiário: Maria Cristina Roque Antunes Barreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático