Expression of bacterial virulence factors and cytokines during in vitro macrophage infection by enteroinvasive Escherichia coli and Shigella flexneri: a comparative study

Autor(es)

Bando, Silvia Y. ^{[1, 2]} ; Moreno, Ana C. R. ^[1] ; Albuquerque, Jose A. T. ^[1] ; Amhaz, Juliana M. K. ^[1] ; Moreira-Filho, Carlos A. ^[2] ; Martinez, Marina B. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Anal Clin & Toxicol, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Dept Pediat, Fac Med, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 6, p. 786-791, SEP 2010.
• Citações Web of Science: 9

Resumo

Enteroinvasive Escherichia coli (EIEC) and Shigellaspp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigellashare many genetic and biochemical similarities, the illness caused by Shigellais more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneriwith cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed thatS. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri. (AU)

• Processo FAPESP: 03/01269-1 - Biologia de escherichia coli enteroinvasora ii: estudo da resposta celular induzida no processo inflamatorio.
• Beneficiário: Marina Baquerizo Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Regular