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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression of bacterial virulence factors and cytokines during in vitro macrophage infection by enteroinvasive Escherichia coli and Shigella flexneri: a comparative study

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Author(s):
Bando, Silvia Y. [1, 2] ; Moreno, Ana C. R. [1] ; Albuquerque, Jose A. T. [1] ; Amhaz, Juliana M. K. [1] ; Moreira-Filho, Carlos A. [2] ; Martinez, Marina B. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Dept Anal Clin & Toxicol, Fac Ciencias Farmaceut, BR-05508000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Pediat, Fac Med, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 105, n. 6, p. 786-791, SEP 2010.
Web of Science Citations: 9
Abstract

Enteroinvasive Escherichia coli (EIEC) and Shigellaspp cause bacillary dysentery in humans by invading and multiplying within epithelial cells of the colonic mucosa. Although EIEC and Shigellashare many genetic and biochemical similarities, the illness caused by Shigellais more severe. Thus, genomic and structure-function molecular studies on the biological interactions of these invasive enterobacteria with eukaryotic cells have focused on Shigella rather than EIEC. Here we comparatively studied the interactions of EIEC and of Shigella flexneriwith cultured J774 macrophage-like cells. We evaluated several phenotypes: (i) bacterial escape from macrophages after phagocytosis, (ii) macrophage death induced by EIEC and S. flexneri, (iii) macrophage cytokine expression in response to infection and (iv) expression of plasmidial (pINV) virulence genes. The results showed thatS. flexneri caused macrophage killing earlier and more intensely than EIEC. Both pathogens induced significant macrophage production of TNF, IL-1 and IL-10 after 7 h of infection. Transcription levels of the gene invasion plasmid antigen-C were lower in EIEC than in S. flexneri throughout the course of the infection; this could explain the diminished virulence of EIEC compared to S. flexneri. (AU)