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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Enhancement of Th1 Lung Immunity Induced by Recombinant Mycobacterium bovis Bacillus Calmette-Guerin Attenuates Airway Allergic Disease

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Autor(es):
Christ, Ana P. [1] ; Rodriguez, Dunia [2] ; Bortolatto, Juliana [2] ; Borducchi, Erica [2] ; Keller, Alexandre [2] ; Mucida, Daniel [2] ; Silva, Joao S. [3] ; Leite, Luciana C. C. [1] ; Russo, Momtchilo [2]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Inst Butantan, Ctr Biotecnol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Imunol, Inst Ciencias Biomed, BR-05508000 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-05508000 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: American Journal of Respiratory Cell and Molecular Biology; v. 43, n. 2, p. 243-252, AUG 2010.
Citações Web of Science: 12
Resumo

Mycobacterium bovis Bacillus Calmette-Guerin (BCG) has been shown to down-regulate experimental allergic asthma, a finding that reinforced the hygiene hypothesis. We have previously found that recombinant BCG (rBCG) strain that express the genetically detoxified Si subunit of pertussis toxin (rBCG-S1PT) exerts an adjuvant effect that enhances Th1 responses against BCG proteins. Here we investigated the effect of this rBCG-S1PT on the classical ovalbumin-induced mouse model of allergic lung disease. We found that rBCG-S1PT was more effective than wild-type BCG in preventing Th2-mediated allergic immune responses. The inhibition of allergic lung disease was not associated with increased concentration of suppressive cytokines or with an increased number of pulmonary regulatory T cells but was positively correlated with the increase in IFN-gamma-producing T cells and T-bet expression in the lung. In addition, an IL-12-dependent mechanism appeared to be important to the inhibition of lung allergic disease. The inhibition of allergic inflammation was found to be restricted to the lung because when allergen challenge was given by the intraperitoneal route, rBCG-S1PT administration failed to inhibit peritoneal allergic inflammation and type 2 cytokine production. Our work offers a nonclassical interpretation for the hygiene hypothesis indicating that attenuation of lung allergy by rBCG could be due to the enhancement of local lung Th1 immunity induced by rBCG-S1PT. Moreover, it highlights the possible use of rBCG strains as multipurpose immunomodulators by inducing specific immunity against microbial products while protecting against allergic asthma. (AU)

Processo FAPESP: 99/05202-1 - Desenvolvimento de vacinas recombinantes contra Streptococcus pneumoniae
Beneficiário:Luciana Cezar de Cerqueira Leite
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 04/14297-6 - Ativação/desativação de macrófagos e linfócitos T CD4+ na asma experimental
Beneficiário:Momtchilo Russo
Linha de fomento: Auxílio à Pesquisa - Temático