Genetic-morphometric variation in Culex quinquefasciatus from Brazil and La Plata, Argentina

Autor(es)

de Morais, Sirlei Antunes ^[1] ; Moratore, Camila ^{[2, 3]} ; Suesdek, Lincoln ^{[2, 3]} ; Marrelli, Mauro Toledo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01255 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Ciencias Biomed, BR-05508 Sao Paulo - Brazil

^[3] Inst Butantan, Secao Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Memórias do Instituto Oswaldo Cruz; v. 105, n. 5, p. 672-676, AUG 2010.
• Citações Web of Science: 20

Resumo

Variation among natural populations of Culex (Culex) quinquefasciatus Say is associated with different vectorial capacities. The species Cx. quinquefasciatus is present in the equatorial, tropical and subtropical zones in the Brazilian territory, with intermediate forms between Cx. quinquefasciatus and Culex pipiens occurring in regions of latitudes around 33 degrees-35 degrees S. Herein, we studied geographically distinct populations of Cx. quinquefasciatus by genetic characterization and analysis of intra-specific wing morphometrics. After morphological analysis, molecular characterization of Cx. quinquefasciatus and intermediate forms was performed by polymerase chain reaction of the polymorphic nuclear region of the second intron of the acetylcholinesterase locus. Additionally, the morphology of adult female wings collected from six locations was analyzed. Wing centroid sizes were significantly different between some geographical pairs. Mean values of R(2)/R(2+3) differed significantly after pairwise comparisons. The overall wing shape represented by morphometric characters could be divided into two main groupings. Our data suggest that Brazilian samples are morphologically and genetically distinct from the Argentinean samples and also indicated a morphological distinction between northern and southern populations of Brazilian Cx. quinquefasciatus. We suggest that wing morphology may be used for preliminary assessment of population structure of Cx. quinquefasciatus in Brazil. (AU)

• Processo FAPESP: 06/02622-5 - Caracterização populacional de Culicidae de interesse médico: aspectos moleculares, cariotípicos e morfométricos
• Beneficiário: Lincoln Suesdek Rocha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/57272-9 - Determinação da variabilidade genética de Culex quinquefasciatus Say, por análise do DNA ribossômico e mitocondrial
• Beneficiário: Sirlei Antunes de Morais
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 05/50225-2 - Desenvolvimento de metodologias alternativas no controle de mosquitos (Diptera: Culicidae) de importância epidemiológica: uso do método ridl (liberação de insetos carregando um gene letal dominante) [... ]
• Beneficiário: Mauro Toledo Marrelli
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores