Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains

Autor(es)

Kahan, Vanessa ^[1] ; Ribeiro, Daniel Araki ^[2] ; Andersen, Monica Levy ^[1] ; Alvarenga, Tathiana Aparecida ^[1] ; Tufik, Sergio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biosci, BR-04024002 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY; v. 107, n. 1, p. 598-602, JUL 2010.
• Citações Web of Science: 10

Resumo

The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. The hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. The same lack of effect was noted in kidney and heart cells of all strains evaluated. In conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose. (AU)

• Processo FAPESP: 98/14303-3 - Center for Sleep Studies
• Beneficiário: Sergio Tufik
• Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 07/01228-4 - Carcinogênese bucal quimicamente induzida pela 4-nitroquinolina 1-óxido em ratos: possíveis biomarcadores envolvidos em sua patogênese
• Beneficiário: Daniel Araki Ribeiro
• Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
• Processo FAPESP: 06/58274-5 - Influência da restrição alimentar no comportamento sexual de ratos machos
• Beneficiário: Tathiana Aparecida Fernandes de Alvarenga
• Linha de fomento: Bolsas no Brasil - Mestrado