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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Sleep Loss Induces Differential Response Related To Genotoxicity in Multiple Organs of Three Different Mice Strains

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Kahan, Vanessa [1] ; Ribeiro, Daniel Araki [2] ; Andersen, Monica Levy [1] ; Alvarenga, Tathiana Aparecida [1] ; Tufik, Sergio [1]
Total Authors: 5
[1] Univ Fed Sao Paulo, Dept Psychobiol, BR-04024002 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Biosci, BR-04024002 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY; v. 107, n. 1, p. 598-602, JUL 2010.
Web of Science Citations: 10

The purpose of the present study was to determine the genetic damage induced by paradoxical sleep deprivation (PSD) in three different male mice strains in peripheral blood, heart, kidney and liver tissues by the single cell gel (comet) assay. Swiss, C57BL/6j and hairless (HRS/j) mice were submitted to PSD by the multiple platform technique for 72 hr, and DNA damage was evaluated. Statistically significant differences in DNA damage were found in blood cells of the Swiss mice strain when compared to negative controls. By contrast, no statistically significant differences were found in the C57BL/6j or hairless mice strains. With regard to the liver, extensive genotoxic effects were found in the Swiss strain. The hairless and C57BL/6j mice strains did not show any signs of genotoxocity in this organ. The same lack of effect was noted in kidney and heart cells of all strains evaluated. In conclusion, our results reveal that sleep deprivation exerted genetic damage in the form of DNA breakage in blood and liver cells of the Swiss mice strain only. This type of approach should be considered when studying noxious activities on genetic apparatus induced by sleep deprivation in mice since the Swiss strain is more suitable for this purpose. (AU)

FAPESP's process: 98/14303-3 - Center for Sleep Studies
Grantee:Sergio Tufik
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 07/01228-4 - Medium-term oral carcinogenesis assay induced by 4-nitroquinoline 1-oxide in rats: putative biomarkers involved into its pathogenesis
Grantee:Daniel Araki Ribeiro
Support type: Research Grants - Young Investigators Grants