Development of secondary palate requires strict regulation of ECM remodeling: sequential distribution of RECK, MMP-2, MMP-3, and MMP-9

Autor(es):Mostrar menos -

Cardoso de Oliveira Demarchi, Ana Claudia ^[1] ; Zambuzzi, Willian Fernando ^[1] ; Silva Paiva, Katiucia Batista ^[2] ; da Silva-Valenzuela, Maria das Gracas ^[3] ; Nunes, Fabio Daumas ^[2] ; Savio Figueira, Rita de Cassia ^[3] ; Sasahara, Regina Maki ^[3] ; Almeida Demasi, Marcos Angelo ^[3] ; Brochado Winnischofer, Sheila Maria ^[3] ; Sogayar, Mari Cleide ^{[3, 4]} ; Granjeiro, Jose Mauro ^{[5, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Biochem, IB, Campinas, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Dept Oral Pathol, Lab Mol Pathol, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Biochem, Inst Chem, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Cell & Mol Therapy Ctr, Sao Paulo - Brazil

^[5] Univ Fed Fluminense, Inst Biol, Dept Cell & Mol Biol, BR-24020150 Niteroi, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cell and Tissue Research; v. 340, n. 1, p. 61-69, APR 2010.
• Citações Web of Science: 15

Resumo

We have evaluated RECK (reversion-inducing-cysteine-rich protein with Kazal motifs), MMP-2 (matrix metalloproteinase-2), MMP-3, and MMP-9 involvement during palate development in mice by using various techniques. Immunohistochemical features revealed the distribution of RECK, MMP-2, and MMP-3 in the mesenchymal tissue and in the midline epithelial seam at embryonic day 13 (E13), MMPs-2, -3, and -9 being particularly expressed at E14 and E14.5. In contrast, RECK was weakly immunostained at these times. Involvement of MMPs was validated by measuring not only their protein expression, but also their activity (zymograms). In situ hybridization signal (ISH) for RECK transcript was distributed in mesenchymal and epithelial regions within palatal shelves at all periods evaluated. Importantly, the results from ISH analysis were in accord with those obtained by real-time polymerase chain reaction. The expression of RECK was found to be temporally regulated, which suggested possible roles in palatal ontogeny. Taken together, our results clearly show that remodeling of the extracellular matrix is finely modulated during secondary palate development and occurs in a sequential manner. (AU)

• Processo FAPESP: 01/10707-7 - Bases moleculares do controle da proliferação celular e origem de neoplasias na era de genômica e proteômica
• Beneficiário: Mari Cleide Sogayar
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 08/53003-9 - Investigação da função da proteína tirosina fosfatase de baixa massa molecular (LMW-PTP) na adesão de osteoblastos e formação óssea
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado