Increased chromosome damage in systemic sclerosis skin fibroblasts

Autor(es)

Martins, E. P. ^[1] ; Fuzzi, H. T. ^[2] ; Kayser, C. ^[1] ; Alarcon, R. T. ^[1] ; Rocha, M. G. C. ^[3] ; Chauffaille, M. L. ; Andrade, L. E. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Rheumatol, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Microbiol, BR-04023062 Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Div Haematol, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCANDINAVIAN JOURNAL OF RHEUMATOLOGY; v. 39, n. 5, p. 398-401, 2010.
• Citações Web of Science: 9

Resumo

Methods: Primary fibroblast cultures were obtained by biopsy from affected and non-affected skin from SSc patients. Control fibroblasts were derived from skin remnants from plastic surgery in healthy adults. The number of micronuclei-bearing cells per 1000 binucleated cells (MN(+) cells/1000 BN) was determined in cultures with and without clastogenic stimulus (bleomycin 3 mu g/mL). Results: Primary cultures from 10 SSc patients (affected and non-affected skin) and nine controls were analysed by two blinded examiners. In the absence of bleomycin, the frequency of MN(+) cells was higher in cultures from affected (14.01 +/- 11.96 MN(+) cells/1000 BN; p = 0.004) and non-affected (15.41 +/- 13.58 MN(+) cells/1000 BN; p = 0.005) skin from SSc patients as compared to fibroblasts from healthy controls (4.74 +/- 3.30 MN(+) cells/1000 BN). In bleomycin-treated cultures, the frequency of MN(+) cells was higher in SSc affected (38.03 +/- 26.14 MN(+) cells/1000 BN; p = 0.041) and non-affected skin (38.47 +/- 17.88 MN(+) cells/1000 BN; p = 0.034) as compared to healthy control fibroblasts (20.54 +/- 13.09 MN(+) cells/1000 BN). There was no difference in the frequency of MN(+) cells in cultures from affected and non-affected skin of SSc patients. Conclusions: This is the first demonstration that dermal fibroblasts from SSc patients present an increased frequency of spontaneous and clastogen-induced micronuclei. Increased clastogenesis seems to be a widespread phenomenon in SSc because fibroblasts from clinically affected and non-affected skin presented the equivalent increased micronuclei counts. (AU)

• Processo FAPESP: 04/00436-4 - Estudo citogenético de fibroblastos de pele acometida e não acometida e linfócitos de sangue periférico de pacientes com esclerose sistêmica e comparação com fibroblastos cutâneos e linfócitos de ...
• Beneficiário: Luiz Eduardo Coelho Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Regular