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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased chromosome damage in systemic sclerosis skin fibroblasts

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Author(s):
Martins, E. P. [1] ; Fuzzi, H. T. [2] ; Kayser, C. [1] ; Alarcon, R. T. [1] ; Rocha, M. G. C. [3] ; Chauffaille, M. L. ; Andrade, L. E. C. [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo, Div Rheumatol, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Dept Microbiol, BR-04023062 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Div Haematol, BR-04023062 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCANDINAVIAN JOURNAL OF RHEUMATOLOGY; v. 39, n. 5, p. 398-401, 2010.
Web of Science Citations: 9
Abstract

Methods: Primary fibroblast cultures were obtained by biopsy from affected and non-affected skin from SSc patients. Control fibroblasts were derived from skin remnants from plastic surgery in healthy adults. The number of micronuclei-bearing cells per 1000 binucleated cells (MN(+) cells/1000 BN) was determined in cultures with and without clastogenic stimulus (bleomycin 3 mu g/mL). Results: Primary cultures from 10 SSc patients (affected and non-affected skin) and nine controls were analysed by two blinded examiners. In the absence of bleomycin, the frequency of MN(+) cells was higher in cultures from affected (14.01 +/- 11.96 MN(+) cells/1000 BN; p = 0.004) and non-affected (15.41 +/- 13.58 MN(+) cells/1000 BN; p = 0.005) skin from SSc patients as compared to fibroblasts from healthy controls (4.74 +/- 3.30 MN(+) cells/1000 BN). In bleomycin-treated cultures, the frequency of MN(+) cells was higher in SSc affected (38.03 +/- 26.14 MN(+) cells/1000 BN; p = 0.041) and non-affected skin (38.47 +/- 17.88 MN(+) cells/1000 BN; p = 0.034) as compared to healthy control fibroblasts (20.54 +/- 13.09 MN(+) cells/1000 BN). There was no difference in the frequency of MN(+) cells in cultures from affected and non-affected skin of SSc patients. Conclusions: This is the first demonstration that dermal fibroblasts from SSc patients present an increased frequency of spontaneous and clastogen-induced micronuclei. Increased clastogenesis seems to be a widespread phenomenon in SSc because fibroblasts from clinically affected and non-affected skin presented the equivalent increased micronuclei counts. (AU)