Stabilization of Endophytic Fungus Cercospora kikuchii Lipase by Spray Drying in the Presence of Maltodextrin and beta-Cyclodextrin

Autor(es)

Costa-Silva, Tales A. ^[1] ; Said, Suraia ^[1] ; Fernandes Souza, Claudia R. ^[1] ; Oliveira, Wanderley P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Drying Technology; v. 28, n. 11, p. 1245-1254, 2010.
• Citações Web of Science: 12

Resumo

In this study the effects of spray-drying conditions on the retention of enzyme activity of lipase produced by the endophytic fungus Cercospora kikuchii have been investigated. Drying runs were carried out in a bench-top spray dryer with a concurrent flow regime. The influence of the variables inlet temperature of drying gas, Tgi (86.4 to 153.6 degrees C); mass flow rate of the enzymatic extract fed to the dryer, Ws (2.63 to 9.36g/min); and concentration of the drying adjuvant added to the extract, ADJ (1.95 to 12.05%), on the spray-drying performance and on product quality was evaluated through experimental planning and regression analysis. The use of maltodextrin, as a stabilizing agent, slightly improved the retention of enzyme activity compared to -cyclodextrin. Statistical optimization of the experimental results allowed the determination of the processing conditions that maximized the retention of the enzymatic activity (RAE), namely, concentration of drying adjuvants of 12.05%, inlet temperature of the drying gas of 153.6 degrees C, and flow rate of the enzymatic extract fed to the dryer of 9.36g/min for the both drying adjuvants investigated. (AU)

• Processo FAPESP: 04/07935-6 - Bioprospecção de fungos: a busca de compostos importantes para o projeto de remédios e enzimas para aplicações farmacêuticas e industriais
• Beneficiário: Jairo Kenupp Bastos
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 08/52732-7 - Caracterização bioquímica e secagem por spray-dryer de lipases produzidas pelo fungo endofítico Cercospora kikuchii
• Beneficiário: Tales Alexandre da Costa e Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado