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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Stabilization of Endophytic Fungus Cercospora kikuchii Lipase by Spray Drying in the Presence of Maltodextrin and beta-Cyclodextrin

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Author(s):
Costa-Silva, Tales A. [1] ; Said, Suraia [1] ; Fernandes Souza, Claudia R. [1] ; Oliveira, Wanderley P. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Dept Ciencias Farmaceut, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Drying Technology; v. 28, n. 11, p. 1245-1254, 2010.
Web of Science Citations: 12
Abstract

In this study the effects of spray-drying conditions on the retention of enzyme activity of lipase produced by the endophytic fungus Cercospora kikuchii have been investigated. Drying runs were carried out in a bench-top spray dryer with a concurrent flow regime. The influence of the variables inlet temperature of drying gas, Tgi (86.4 to 153.6 degrees C); mass flow rate of the enzymatic extract fed to the dryer, Ws (2.63 to 9.36g/min); and concentration of the drying adjuvant added to the extract, ADJ (1.95 to 12.05%), on the spray-drying performance and on product quality was evaluated through experimental planning and regression analysis. The use of maltodextrin, as a stabilizing agent, slightly improved the retention of enzyme activity compared to -cyclodextrin. Statistical optimization of the experimental results allowed the determination of the processing conditions that maximized the retention of the enzymatic activity (RAE), namely, concentration of drying adjuvants of 12.05%, inlet temperature of the drying gas of 153.6 degrees C, and flow rate of the enzymatic extract fed to the dryer of 9.36g/min for the both drying adjuvants investigated. (AU)

FAPESP's process: 04/07935-6 - Bioprospection in fungi: the search of lead compounds for drug design and enzymes for pharmaceutical and industrial applications
Grantee:Jairo Kenupp Bastos
Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
FAPESP's process: 08/52732-7 - Biochemical characterization and spray drying of lipases produced by the endophytic fungus Cercospora kicuchii
Grantee:Tales Alexandre da Costa e Silva
Support Opportunities: Scholarships in Brazil - Master