Interleukin-10 but not Transforming Growth Factor beta inhibits murine activated macrophages Paracoccidioides brasiliensis killing: Effect on H2O2 and NO production

Autor(es)

Moreira, Ana Paula ^[1] ; Dias-Melicio, Luciane Alarcao ^[1] ; Victoriano Campos Soares, Angela Maria ^[1]

Afiliação do(s) autor(es)

^[1] UNESP Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Immunol, BR-18618000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Cellular Immunology; v. 263, n. 2, p. 196-203, 2010.
• Citações Web of Science: 27

Resumo

Paracoccidioidomycosis is caused by the thermally dimorphic fungus Paracoccidioides brasiliensis (P brasiliensis). Most often, this mycosis runs as a chronic progressive course affecting preferentially the lungs. In vitro fungicidal activity against a high virulent strain of P brasiliensis by murine peritoneal macrophages preactivated with IFN-gamma or TNF-alpha is high and correlates with increased NO and H(2)O(2) production. Within this context, the purpose of this work was to study the role of suppressor cytokines, such as IL-10 and TGF-beta, in this process. Incubation of either IFN-gamma or TNF-alpha with IL-10 inhibits fungicidal activity of these cells However, TGF-beta had no effect on fungicidal activity of IFN-gamma or TNF-alpha-activated macrophages. The suppression of fungicidal activity by IL-10 correlated with the inhibition of NO and H(2)O(2) production supporting the involvement of these metabolites in P brasiliensis killing These results suggest that IL-10 production in vivo could represent an evasion mechanism of the fungus to avoid host immune response (C) 2010 Elsevier Inc All rights reserved. (AU)

• Processo FAPESP: 00/04876-8 - Atividade fungicida contra o Paracoccidioides brasiliensis de macrófagos peritoneais murinos ativados por citocinas: papel dos metabólitos do oxigênio e nitrogênio; papel das citocinas supressoras IL-10 e TGF-beta
• Beneficiário: Ana Paula Moreira Serezani
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica