Extension of the core map of common bean with EST-SSR, RGA, AFLP, and putative functional markers

Autor(es)

Hanai, Luiz Ricardo ^[1] ; Santini, Luciane ^[1] ; Aranha Camargo, Luis Eduardo ^[1] ; Pelegrinelli Fungaro, Maria Helena ^[2] ; Gepts, Paul ^[3] ; Tsai, Siu Mui ^[4] ; Carneiro Vieira, Maria Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[2] Univ Estadual Londrina, Ctr Ciencias Biol, BR-86055900 Londrina, PR - Brazil

^[3] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 - USA

^[4] Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13416000 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR BREEDING; v. 25, n. 1, p. 25-45, JAN 2010.
• Citações Web of Science: 54

Resumo

Microsatellites and gene-derived markers are still underrepresented in the core molecular linkage map of common bean compared to other types of markers. In order to increase the density of the core map, a set of new markers were developed and mapped onto the RIL population derived from the `BAT93' x `Jalo EEP558' cross. The EST-SSR markers were first characterized using a set of 24 bean inbred lines. On average, the polymorphism information content was 0.40 and the mean number of alleles per locus was 2.7. In addition, AFLP and RGA markers based on the NBS-profiling method were developed and a subset of the mapped RGA was sequenced. With the integration of 282 new markers into the common bean core map, we were able to place markers with putative known function in some existing gaps including regions with QTL for resistance to anthracnose and rust. The distribution of the markers over 11 linkage groups is discussed and a newer version of the common bean core linkage map is proposed. (AU)

• Processo FAPESP: 08/52269-5 - Mapeamento de genes análogos de resistência a patógenos em feijão (Phaseolus vulgaris L.)
• Beneficiário: Luciane Santini Gazaffi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/54732-4 - Mapeamento de genes analogos de resistencia a patogenos em feijao (phaseolus vulgaris l.)
• Beneficiário: Maria Lúcia Carneiro Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/13547-9 - Mapeamento de marcadores ssr est-derivados e aflp em feijao (phaseolus vulgaris l.).
• Beneficiário: Maria Lúcia Carneiro Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 04/07614-5 - Mapeamento de marcadores SSR EST-derivados e AFLP em feijão (Phaseolus vulgaris L.)
• Beneficiário: Luiz Ricardo Hanai
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto