| Texto completo | |
| Autor(es): |
Simionato, Ana Valeria C.
[1, 2, 3]
;
Silva-Stenico, Maria Estela
[4]
;
Tsai, Siu Mui
[4]
;
Carrilho, Emanuel
[1, 3]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Estadual Campinas, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Grp Toxicol Alimentos & Farmacos, BR-13083970 Campinas, SP - Brazil
[3] Univ Sao Paulo, Grp Bioanalit Microfabricacao & Separacoes, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP - Brazil
[4] Univ Sao Paulo, Lab Biol Celular & Mol, Ctr Energia Nucl Agr, BR-13416000 Piracicaba, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of the Brazilian Chemical Society; v. 21, n. 4, p. 635-641, 2010. |
| Citações Web of Science: | 1 |
| Resumo | |
Siderophore molecules from grapevine Xylella fastidiosa were investigated. Such metabolites sequester iron, an essential element, from the host, making them a potential factor of pathogenicty. In an iron-limited medium, siderophores were detected in culture plates of X. fastidiosa containing the complex Chromeazurol S (CAS). A combination of different instrumental analyses was used for siderophore(s) characterization, such as: immobilized metal affinity chromatography (IMAC), micelar electrokinetic capillary chromatography (MEKC) and electrospray-mass spectrometry (ESI-MS). The results show that grapevine X. fastidiosa produced siderophore(s), as confirmed by the CAS plate assay. The extraction of the compound(s) using IMAC with immobilized Fe3+ was important for analyte purification. The chelator was not separated by capillary zone electrophoresis, indicating the possibility of a neutral compound under the investigated pHs. MEKC runs presented a different peak (when compared to the control analysis) which represented a slightly hydrophobic compound. Mass spectrometry showed that the compound(s) may have a relative molecular mass within the expected range for siderophore molecules, such as 875, 1004 and 1092 Da. (AU) | |
| Processo FAPESP: | 00/12207-9 - Isolamento e identificacao de compostos quelantes de ferro da bacteria "xylella fastidiosa" por eletroforese capilar acoplada a espectrometria de massas (ce-ms). |
| Beneficiário: | Ana Valéria Colnaghi Simionato |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 08/57805-2 - Instituto de Bioanalítica |
| Beneficiário: | Lauro Tatsuo Kubota |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 99/09177-1 - Diversidade de microrganismos endofíticos e seu potencial biotecnológico |
| Beneficiário: | Itamar Soares de Melo |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Temático |